caa a22 4500 44580131X CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1883-4 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1883-4 HPLC-MS-MS for the Simultaneous Determination of Atorvastatin and Amlodipine in Plasma of Hypertensive Patients [Elektronische Daten] [Qi Yu, Zhe-Yi Hu, Fan-Yuan Zhu, Jin-Hui Zhu, Li-Li Wan, Yan Li, Cheng Guo] Combination drug products containing amlodipine and atorvastatin are widely marketed and used in the treatment of concomitant hypertension and dyslipidemia. A rapid, simple and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method for determination of atorvastatin and amlodipine in plasma of hypertensive patients has been developed and validated to be used for therapeutic drug monitoring. The plasma samples were subjected to methanol protein precipitation. Chromatographic separation was performed on a C18 column using a gradient elution. The mobile phase consisted of 0.1% of formic acid in water and 0.1% of formic acid in acetonitrile and was pumped at a flow rate of 0.4mLmin−1. Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode. The calibration curves were linear over the range of 0.46-1,000ngmL−1. The intra- and inter-day precisions were within 12.2%, while the accuracy ranged from 92.7 to 108.1%. The validated LC-MS-MS method was successfully applied for the determination of atorvastatin and amlodipine in plasma of hypertensive patients. Springer-Verlag, 2011 High performance liquid chromatography nationallicence Tandem mass spectrometry nationallicence Atorvastatin nationallicence Amlodipine nationallicence Plasma samples nationallicence Yu Qi Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut Hu Zhe-Yi Shanghai Center for DMPK Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China aut Zhu Fan-Yuan Center of Renal Transplantation, Changzheng Hospital, Second Military Medical University, Shanghai, China aut Zhu Jin-Hui Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut Wan Li-Li Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut Li Yan Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut Guo Cheng Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 257-262 0009-5893 73:3-4<257 2011 73 10337 https://doi.org/10.1007/s10337-010-1883-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1883-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Qi Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut NATIONALLICENCE 700 1- Hu Zhe-Yi Shanghai Center for DMPK Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China aut NATIONALLICENCE 700 1- Zhu Fan-Yuan Center of Renal Transplantation, Changzheng Hospital, Second Military Medical University, Shanghai, China aut NATIONALLICENCE 700 1- Zhu Jin-Hui Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut NATIONALLICENCE 700 1- Wan Li-Li Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut NATIONALLICENCE 700 1- Li Yan Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut NATIONALLICENCE 700 1- Guo Cheng Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 257-262 0009-5893 73:3-4<257 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer