caa a22 4500 445801344 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10337-010-1879-0 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1879-0 A Sensitive LC-MS-MS Method for Simultaneous Quantification of Two Structural Isomers, Hyperoside and Isoquercitrin: Application to Pharmacokinetic Studies [Elektronische Daten] [Chunling Zhou, Yang Liu, Dan Su, Guanghui Gao, Xiaomian Zhou, Lingling Sun, Xinguo Ba, Xiaohui Chen, Kaishun Bi] We report the development and validation of a rapid, specific, and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method for analysis and pharmacokinetic study, in rats, of hyperoside and isoquercitrin, two bioactive structural isomers present in the leaves of Apocynum venetum L. After simple deproteinization by addition of acetonitrile, the analytes were separated on a C18 column. Detection was by tandem mass spectrometry in multiple reaction monitoring mode. The method was linear over the concentration range 3.9-195ngmL−1 for both hyperoside and isoquercitrin. Intra-day and inter-day precision for both hyperoside and isoquercitrin were <13.1%, and relative errors were all within 7.1% at all QC levels. The method was used to study the pharmacokinetic performance of the compounds after oral administration of an extract of Apocynum venetum L. leaves to rats. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence Pharmacokinetics nationallicence Hyperoside nationallicence Isoquercitrin nationallicence Zhou Chunling Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Liu Yang Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut Su Dan The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, China aut Gao Guanghui Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut Zhou Xiaomian Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Sun Lingling Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut Ba Xinguo Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut Chen Xiaohui Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Bi Kaishun Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 353-359 0009-5893 73:3-4<353 2011 73 10337 https://doi.org/10.1007/s10337-010-1879-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1879-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Chunling Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Liu Yang Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut NATIONALLICENCE 700 1- Su Dan The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, China aut NATIONALLICENCE 700 1- Gao Guanghui Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut NATIONALLICENCE 700 1- Zhou Xiaomian Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sun Lingling Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut NATIONALLICENCE 700 1- Ba Xinguo Liaoning Provincial Institute for Control of Food and Drugs, 46 Guihe Street, 110023, Shenyang, China aut NATIONALLICENCE 700 1- Chen Xiaohui Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Bi Kaishun Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/3-4(2011-02-01), 353-359 0009-5893 73:3-4<353 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer