caa a22 4500 445801409 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1950-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1950-5 Simultaneous Ion-Pair LC Analysis of Ephedrine and Pseudoephedrine in Rat Liver Microsomal Incubations: Application to In-Vitro Metabolism [Elektronische Daten] [Jingling Tang, Xiaoyan Zhou, Hongyu Ji, Na Xu, Hongmei Liu, Gongzan Lan, Linhua Wu] (−)-Ephedrine (ephedrine, EPH) and (+)-ephedrine (pseudoephedrine, PEPH) are metabolized by the liver, but the species of hepatocyte cytochrome P450 (CYP450) responsible is not yet clear. To investigate which subtype of CYP450 is involved in the metabolism of EPH and PEPH, a rapid and reliable reversed-phase ion-pair liquid chromatographic method for simultaneous analysis of EPH and PEPH in rat liver microsomes has been established and validated. Matrine was selected as a suitable internal standard (IS) for calibration. After liquid-liquid extraction of liver microsomal samples with methyl tert-butyl ether, EPH and PEPH were separated on a C18 reversed-phase column (200mm×4.6mm, 5μm) with methanol-0.5% sodium dodecyl sulfate-phosphoric acid-triethylamine 60:40:1.25:1 (v/v) as mobile phase at a flow rate of 1.0mLmin. Detection was by UV absorbance at 210.5nm. For both EPH and PEPH, calibration curves were linear over the range 1.5-60.0μgmL−1, the limit of quantification was 1.5μgmL−1, and intra-day and inter-day variability was <10.0%. Average extraction recovery of the two analytes was >73%. The validated method was successfully used to study the in-vitro metabolism of EPH and PEPH. In rat liver microsomes induced by dexamethasone, enzyme activity in the metabolism of EPH and PEPH was higher than that for metabolism of phenobarbital and β-naphthoflavone. Springer-Verlag, 2011 Column ion-pair liquid chromatography nationallicence Ephedrine nationallicence Pseudoephedrine nationallicence Rat liver microsomes nationallicence Metabolism nationallicence Tang Jingling Department of Pharmaceutics, School of Pharmacy, Harbin Medical University, 150086, Harbin, China aut Zhou Xiaoyan Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Ji Hongyu Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Xu Na Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Liu Hongmei Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Lan Gongzan Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Wu Linhua Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 761-766 0009-5893 73:7-8<761 2011 73 10337 https://doi.org/10.1007/s10337-011-1950-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1950-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tang Jingling Department of Pharmaceutics, School of Pharmacy, Harbin Medical University, 150086, Harbin, China aut NATIONALLICENCE 700 1- Zhou Xiaoyan Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 700 1- Ji Hongyu Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 700 1- Xu Na Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 700 1- Liu Hongmei Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 700 1- Lan Gongzan Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 700 1- Wu Linhua Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, 157 Baojian Road, 150086, Harbin, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 761-766 0009-5893 73:7-8<761 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer