caa a22 4500 445801417 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1963-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1963-0 Quantitative LC Analysis of Cyclosporine A in Ocular Samples [Elektronische Daten] [Bizhan Malaekeh-Nikouei, Toka Banaee, Javad Aghamohammadian, Navid Mosallaei, Seyed Mohajeri] An isocratic reversed-phase HPLC method with ultraviolet detection at 205nm has been developed for analysis of cyclosporine A (CyA) in rabbit ocular samples. Neither internal standard nor extraction was needed for sample preparation. Acetonitrile (ACN; 1mL) was added to 250μL aqueous and vitreous samples to precipitate proteins. The supernatant was dried and the residue was reconstituted in mobile phase and injected for HPLC analysis. Chromatography was performed on an octadecyl silane-A (ODSA) C18 (4.6×250mm, 5μm) column. The column temperature was fixed at 70°C and the mobile phase was ACN 65%, methanol 20% and water 15% at a flow rate of 1.5mLmin−1. The calibration curve for CyA in rabbit ocular samples was linear over the concentration range 0.2 and 10μgmL−1 with a correlation coefficient of 0.9992. Intra-day and inter-day precision were 4.61-7.83% and 5.27-10.70%, respectively. Intra-day and inter-day accuracy were 89.2-108% and 83.4-111%, respectively. The limits of detection (LOD) and quantification (LOQ) were 5.7 and 38ngmL−1, respectively. The method was successfully used for analysis of CyA in real aqueous and vitreous humor samples from New Zealand albino rabbits. The method is therefore suitable for analysis of CyA in ocular samples. Springer-Verlag, 2011 Column liquid chromatography nationallicence Ocular samples nationallicence Cyclosporine A nationallicence Malaekeh-Nikouei Bizhan Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut Banaee Toka Khatam-al-Anbia Eye Hospital and Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran aut Aghamohammadian Javad Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut Mosallaei Navid Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut Mohajeri Seyed Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 817-821 0009-5893 73:7-8<817 2011 73 10337 https://doi.org/10.1007/s10337-011-1963-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1963-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malaekeh-Nikouei Bizhan Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut NATIONALLICENCE 700 1- Banaee Toka Khatam-al-Anbia Eye Hospital and Eye Research Center, Mashhad University of Medical Sciences, Mashhad, Iran aut NATIONALLICENCE 700 1- Aghamohammadian Javad Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut NATIONALLICENCE 700 1- Mosallaei Navid Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut NATIONALLICENCE 700 1- Mohajeri Seyed Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 817-821 0009-5893 73:7-8<817 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer