caa a22 4500 445801433 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1926-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1926-5 A Combined Peritoneal Macrophage/Cell Membrane Chromatography and Offline GC-MS Method for Screening Anti-Inflammatory Components from Chinese Traditional Medicine Houttuynia cordata Thunb [Elektronische Daten] [Weifeng Li, Xiaofeng Niu, Ping Zhou, Miao Li, Langchong He] The aim of this study was to screen for the anti-inflammatory components from Houttuynia cordata Thunb (HCT) using an analytical method combining cell membrane chromatography (CMC) with gas chromatography-mass spectrometry (GC-MS). The stationary phase of the CMC employed mouse peritoneal macrophage (PM) cell membranes. We investigated the performance of the PM/CMC-offline-GC-MS method using hydrocortisone (HC) as standard. The method was then applied to the identification of anti-inflammatory components in extracts of HCT. The major component retained by CMC was identified as methyl nonyl ketone (MNK) by GC-MS. In vitro experiments revealed that MNK was able to inhibit LPS-induction of TNF-α, NO, and H2O2 production in a dose-dependent manner. IC50 values were 3.3, 4.1, and 3.6μgmL−1, respectively. The PM/CMC-offline-GC-MS method is an effective screening system for the rapid detection, enrichment, and identification of target components from complex samples. Springer-Verlag, 2011 Cell membrane chromatography nationallicence Gas chromatography-mass spectrometry nationallicence Methyl nonyl ketone nationallicence Peritoneal macrophage nationallicence Anti-inflammatory nationallicence Li Weifeng School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut Niu Xiaofeng School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut Zhou Ping School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut Li Miao School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut He Langchong School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 673-680 0009-5893 73:7-8<673 2011 73 10337 https://doi.org/10.1007/s10337-011-1926-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1926-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Weifeng School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut NATIONALLICENCE 700 1- Niu Xiaofeng School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut NATIONALLICENCE 700 1- Zhou Ping School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut NATIONALLICENCE 700 1- Li Miao School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut NATIONALLICENCE 700 1- He Langchong School of Medicine, Xi'an Jiaotong University, No. 76 Western Yanta Road, 710061, Xi'an, Shaanxi, PR China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 673-680 0009-5893 73:7-8<673 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer