caa a22 4500 445801441 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1937-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1937-2 Simultaneous Estimation of Vinpocetine and Its Metabolite, Apovincaminic Acid, in Beagle Plasma by LC-MS-MS [Elektronische Daten] [Yuan Yang, Kun Lou, Wei Gong, Xing-Guo Mei, Kai-Chun Zhang] A method was developed to determine vinpocetine and its metabolite, apovincaminic acid, in beagle plasma by LC-MS-MS. After protein precipitation with methanol, the supernatant of the sample was concentrated and injected into an Agilent Zorbax XDB-C18 column. The sample was separated by a mobile phase consisting of acetonitrile and 0.2% formic acid solution, and the reading was determined on an Agilent 6410 Triple Quad Tandem mass spectrometer in multiple reaction monitoring mode with the following transitions: m/z 351.5→280.2/266.3 for vinpocetine, 323.2→236.1/280.2 for apovincaminic acid, and 411.2→191.1 for the internal standard. The intra- and inter-day variances were less than 15% (RSD%), and average recoveries were higher than 80%. The linearity ranges (LR) between 0.1 and 20.0ngmL−1 for vinpocetine (r 2=0.9980) and between 1.0 and 200.0ngmL−1 for apovincaminic acid (r 2=0.9995) were established. In summary, this method is sensitive, specific, and appropriate for in vivo study of various dosage forms of vinpocetine. Springer-Verlag, 2011 Column liquid chromatography-mass spectrometry nationallicence Multiple reaction monitoring nationallicence Apovincaminic acid nationallicence Vinpocetine nationallicence Yang Yuan Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, RuiWangFen 12th, HaiDian district, 100093, Beijing, People's Republic of China aut Lou Kun Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut Gong Wei Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut Mei Xing-Guo Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut Zhang Kai-Chun Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, RuiWangFen 12th, HaiDian district, 100093, Beijing, People's Republic of China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 775-779 0009-5893 73:7-8<775 2011 73 10337 https://doi.org/10.1007/s10337-011-1937-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1937-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Yuan Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, RuiWangFen 12th, HaiDian district, 100093, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Lou Kun Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gong Wei Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Mei Xing-Guo Beijing Institute of Pharmacology and Toxicology, TaiPing Road 27th, HaiDian district, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Kai-Chun Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, RuiWangFen 12th, HaiDian district, 100093, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 775-779 0009-5893 73:7-8<775 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer