caa a22 4500 445801468 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1945-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1945-2 Determination of Vecuronium Bromide in Pharmaceuticals: Development, Validation and Comparative Study of HPLC and CZE Analytical Methods [Elektronische Daten] [Pedro García, Fabio Gomes, Maria Santoro, Érika Kedor-Hackmann, Jose Quero, Alberto Montón, Guillermo Montoya, Mercedes Cabrera] Vecuronium bromide is a neuromuscular blocking agent used for anesthesia to induce skeletal muscle relaxation. HPLC and CZE analytical methods were developed and validated for the quantitative determination of vecuronium bromide. The HPLC method was achieved on an amino column (Luna 150×4.6mm, 5μm) using UV detection at 205nm. The mobile phase was composed of acetonitrile:water containing 25.0mmolL−1 of sodium phosphate monobasic (50:50 v/v), pH 4.6 and flow rate of 1.0mLmin−1. The CZE method was achieved on an uncoated fused-silica capillary (40.0cm total length, 31.5cm effective length and 50μm i.d.) using indirect UV detection at 230nm. The electrolyte comprised 1.0mmolL−1 of quinine sulfate dihydrate at pH 3.3 and 8.0% of acetonitrile. The results were used to compare both techniques. No significant differences were observed (p>0.05). Springer-Verlag, 2011 Capillary zone electrophoresis nationallicence Column liquid chromatography nationallicence Vecuronium bromide nationallicence García Pedro Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut Gomes Fabio Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut Santoro Maria Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut Kedor-Hackmann Érika Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut Quero Jose Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut Montón Alberto Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut Montoya Guillermo Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut Cabrera Mercedes Andaluz Service of Health, University of Granada, P.O. Box 18071, Granada, GR, Spain aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 799-805 0009-5893 73:7-8<799 2011 73 10337 https://doi.org/10.1007/s10337-011-1945-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1945-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- García Pedro Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Gomes Fabio Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Santoro Maria Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Kedor-Hackmann Érika Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, P.O. Box 66355, 05508-900, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Quero Jose Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut NATIONALLICENCE 700 1- Montón Alberto Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut NATIONALLICENCE 700 1- Montoya Guillermo Department of Analytical Chemistry, Faculty of Sciences, University of Granada, P.O. Box 18071, Granada, GR, Spain aut NATIONALLICENCE 700 1- Cabrera Mercedes Andaluz Service of Health, University of Granada, P.O. Box 18071, Granada, GR, Spain aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 799-805 0009-5893 73:7-8<799 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer