caa a22 4500 445801484 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1957-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-1957-y GC Analysis of Amino Acids Using Trifluoroacetylacetone and Ethyl Chloroformate as Derivatizing Reagents in Skin Samples of Psoriatic and Arsenicosis Patients [Elektronische Daten] [Muhammad Khuhawar, Subhan Majidano] The separation and determination of 19 amino acids were examined using two stages derivatization with trifluoroacetylacetone and ethyl chloroformate from the column HP-5 (30m×0.32mm id) with film thickness 0.25μm at an initial column temperature 100°C for 2min with ramping of 20°C min−1 up to 250°C with nitrogen flow rate of 3mLmin−1. The detection was performed by flame ionization detector. Total separation time was 10min. The separation was repeatable with relative standard deviation (RSD) (n=5) within 1.5-1.9 and 1.3-1.7% in terms of retention time and peak height/peak area, respectively. The method was applied for the determination of amino acids from skin samples of psoriatic patients (n=6), arsenicosis patients (n=5) and normal subjects (n=9) and variation in the contents of the amino acids was noted. The RSDs for the determination were obtained within 3%. Springer-Verlag, 2011 Gas chromatography nationallicence Amino acids nationallicence Trifluoroacetylacetone nationallicence Ethyl chloroformate nationallicence Psoriasis nationallicence Arsenicosis nationallicence Khuhawar Muhammad Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan aut Majidano Subhan Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 701-708 0009-5893 73:7-8<701 2011 73 10337 https://doi.org/10.1007/s10337-011-1957-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1957-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khuhawar Muhammad Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan aut NATIONALLICENCE 700 1- Majidano Subhan Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 701-708 0009-5893 73:7-8<701 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer