caa a22 4500 445801492 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1961-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1961-2 Evaluation of Nonionic Block Polymer Surfactants in Maize Root Proteome Extraction within Water-Organic Solvent Phases [Elektronische Daten] [Hongbo Ma, Yufeng Hu, Futong Yu, Xueqin Ren, Fusuo Zhang] In the present work, six nonionic block copolymer surfactants consisting of poly(ethylene glycol) (PEG), polyethylene (PE), and poly(propylene glycol) (PPG) were exploited to extract total proteins from maize (Zea mays L.) roots within a water-organic solvent system. After the treatment, proteins were partitioned into aqueous phase, interphase, and organic phase. The total yield was increased by up to 30% after adding PE-PEG block polymer surfactant compared with control. Two-dimensional electrophoresis (2-DE) was further used to evaluate proteins in water phase and interphase; the results revealed that the spot numbers and density were all improved, and well-focused electrophoretic patterns were achieved with higher density and without excess Joule heating problem after adding nonionic block polymer surfactants. Among the six investigated block polymers, PE-PEG extracted the largest amount of proteins. Springer-Verlag, 2011 Electrophoresis nationallicence Block polymer surfactant nationallicence Maize root nationallicence Proteome extraction nationallicence Two phases nationallicence Ma Hongbo Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut Hu Yufeng Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut Yu Futong Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut Ren Xueqin Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut Zhang Fusuo Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 681-690 0009-5893 73:7-8<681 2011 73 10337 https://doi.org/10.1007/s10337-011-1961-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1961-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Hongbo Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Hu Yufeng Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yu Futong Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Ren Xueqin Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Fusuo Department of Plant Nutrition, College of Resources and Environmental Sciences, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 681-690 0009-5893 73:7-8<681 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer