caa a22 4500 445801514 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-010-1881-6 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1881-6 An Optimized Ion-Pair HPLC Method for Simultaneous Analysis of Nucleoside Triphosphate Levels in Hepatoma Cell Line [Elektronische Daten] [Jing Jia, Hai Zhang, Liang Zhao, Zhen-yu Zhu, Guo-qing Zhang, Yi-feng Chai] An improved ion-pair HPLC method was developed for the simultaneous determination of ribonucleoside triphosphates and their corresponding deoxyribonucleoside triphosphates in HepG2 cell extracts. HPLC conditions, flow rate and column temperature were optimized and good linearity (r 2>0.9993) was obtained over the investigated concentration ranges. Reproducibility was evaluated by intra- and inter-day assays and RSD values were below 5.39%. Recoveries ranged from 98.2±3.49% to 103.1±1.75%, respectively. Finally, the method was successfully applied to the analysis of eight compounds present in HepG2 cell extracts. Springer-Verlag, 2011 Ion-pair HPLC nationallicence Nucleoside triphosphate nationallicence HepG2 nationallicence Jia Jing Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Zhang Hai Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut Zhao Liang Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut Zhu Zhen-yu Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Zhang Guo-qing Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut Chai Yi-feng Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 755-759 0009-5893 73:7-8<755 2011 73 10337 https://doi.org/10.1007/s10337-010-1881-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1881-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Jing Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hai Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Liang Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Zhen-yu Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Guo-qing Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 200438, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chai Yi-feng Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 755-759 0009-5893 73:7-8<755 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer