caa a22 4500 445801530 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1947-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1947-0 An LC-MS-MS Method for Quantification of CMDCK: A Novel Anti-HIV Agent in Rat Plasma and Its Application to a Pharmacokinetic Study [Elektronische Daten] [Weili Kong, Xiaomei Zhuang, Hua Li, Xiaofeng Wang, Lan Xie] A simple and sensitive LC-MS-MS method was developed and validated to quantify CMDCK, a novel non-nucleoside reverse transcriptase inhibitor in rat plasma. A simple protein precipitation with acetonitrile was used to pretreat plasma samples. Chromatographic separation was carried out on a Hypersil GOLD-C18 (50mm×2.1mm, 5μm) column with the mobile phase consisting of acetonitrile and 0.1% formic acid solution (60:40 v/v) at a flow rate of 0.2mLmin−1. A triple-quadrupole tandem mass spectrometer with ESI source was operated in the positive ion mode. Quantitative analysis was conducted in the selected reaction monitoring mode with precursor/product ion transitions of m/z 698/500 and 693/495, respectively for CMDCK and IS. The calibration curves were linear over the concentration range of 5-2,000ngmL−1 with the lower limit of quantification of 5ngmL−1. The intra- and inter-day precisions and accuracies were satisfactory, with the coefficient of variation and the relative error being less than 13.2 and 3.38%, respectively. The recovery of CMDCK in plasma ranged from 84.6±6.5 to 100.8±9.3%. This LC-MS-MS method was successfully used as a new approach for the preclinical pharmacokinetics study of CMDCK in rat. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence CMDCK nationallicence Pharmacokinetics nationallicence Kong Weili Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Zhuang Xiaomei Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Li Hua Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Wang Xiaofeng Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Xie Lan Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 649-657 0009-5893 73:7-8<649 2011 73 10337 https://doi.org/10.1007/s10337-011-1947-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1947-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kong Weili Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhuang Xiaomei Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Hua Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiaofeng Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xie Lan Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 649-657 0009-5893 73:7-8<649 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer