caa a22 4500 445801549 CHVBK 20180317145142.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1946-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1946-1 Quantitative Determination of Lobeline Hydrochloride in Rabbit Plasma by LC-MS-MS and Its Application [Elektronische Daten] [Zhennan Zhang, Gexin Dai, Yaozhen Ye, Zhe Sun, Meilfei Lu, Jianshe Ma, Xianqin Wang] A sensitive and selective liquid chromatography tandem mass spectrometry method for quantitative determination of lobeline hydrochloride in rabbit plasma was developed and validated. After addition of triazolam as internal standard, protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 338.1→315.8 for lobeline hydrochloride and m/z 342.9→308.0 for the IS. Calibration plots were linear over the range of 2-500ngmL−1 for lobeline hydrochloride in plasma. Lower limit of quantitation for lobeline hydrochloride was 2ngmL−1. Mean recovery of lobeline hydrochloride from plasma was in the range 97.5-102.3%. RSD of intra-day and inter-day precision were both <9%. This developed method is successfully used in pharmacokinetic study of lobeline hydrochloride in rabbit. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence Lobeline hydrochloride nationallicence Pharmacokinetics nationallicence Plasma nationallicence Zhang Zhennan Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut Dai Gexin The First Affiliated Hospital, Wenzhou Medical College, 325000, Wenzhou, China aut Ye Yaozhen Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut Sun Zhe Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut Lu Meilfei Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut Ma Jianshe Function Experiment Teaching Center, Wenzhou Medical College, 325035, Wenzhou, China aut Wang Xianqin Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 781-786 0009-5893 73:7-8<781 2011 73 10337 https://doi.org/10.1007/s10337-011-1946-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1946-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Zhennan Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Dai Gexin The First Affiliated Hospital, Wenzhou Medical College, 325000, Wenzhou, China aut NATIONALLICENCE 700 1- Ye Yaozhen Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Sun Zhe Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Lu Meilfei Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Ma Jianshe Function Experiment Teaching Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Wang Xianqin Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 781-786 0009-5893 73:7-8<781 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer