caa a22 4500 44580159X CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1919-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1919-4 LC-MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase [Elektronische Daten] [Karen Fried, Paul Nolan Jr., Marietta Anthony, Raymond Woosley, Marlene Freeman] A sensitive liquid chromatography-mass spectrometry method for the simultaneous determination of sertraline (SER) and its major metabolite norsertraline (NOR) from serum was developed and validated in the context of a pharmacokinetic study in pregnant women. The separations were achieved on a silica column with a non-aqueous polar mobile phase consisting of acetonitrile, methanol and ammonium acetate at a flow rate of 0.5mLmin−1. The concentrations were measured using a single quadruple mass spectroscopic detector supplied with atmospheric pressure ionization electrospray. Sample preparation consisted of a simple liquid-liquid procedure. The detector was set in selective ion mode for each compound of interest, 306m/z for SER and 275m/z for NOR. Calibration curves were generated by least square linear regression for concentration of 5-160ngmL−1 for SER and from 10 to 320ngmL−1 for NOR. The curves for both compounds of interest were linear, with correlation coefficients r 2≥0.999. Springer-Verlag, 2011 Column liquid chromatography-mass spectrometry nationallicence NA-HILIC nationallicence Serum nationallicence Sertraline nationallicence Norsertraline nationallicence Fried Karen Department of Pharmacology and Toxicology, University of Arizona College of Pharmacy, Tucson, AZ, USA aut Nolan Jr. Paul Department of Pharmacy Practice and Science, University of Arizona College of Pharmacy, Tucson, AZ, USA aut Anthony Marietta The Critical Path Institute, Tucson, AZ, USA aut Woosley Raymond The Critical Path Institute, Tucson, AZ, USA aut Freeman Marlene Department of Psychiatry, Massachusetts General Hospital, Boston, MA, USA aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 749-754 0009-5893 73:7-8<749 2011 73 10337 https://doi.org/10.1007/s10337-011-1919-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1919-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fried Karen Department of Pharmacology and Toxicology, University of Arizona College of Pharmacy, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Nolan Jr Paul Department of Pharmacy Practice and Science, University of Arizona College of Pharmacy, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Anthony Marietta The Critical Path Institute, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Woosley Raymond The Critical Path Institute, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Freeman Marlene Department of Psychiatry, Massachusetts General Hospital, Boston, MA, USA aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 749-754 0009-5893 73:7-8<749 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer