caa a22 4500 445801611 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1951-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1951-4 Determination of Revaprazan in Human Plasma by LC-MS-MS and Application to a Pharmacokinetic Study in Chinese Volunteers [Elektronische Daten] [Peng Yu, Hang Cheng, Xin Guo, Zhi Liu, Xiao Li, Zeneng Cheng] A sensitive, specific, and rapid liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed for determination of revaprazan in human plasma. Plasma samples were simply treated with methanol to precipitate, and then isolated supernatants were directly injected into the LC-ESI-MS-MS system. A Thermo Hypurity C18 column (150×2.1mm, 5μm) with mobile phase of methanol-water (70:30, v/v) containing 0.05% formic acid was used for chromatographic separation. Mass-spectrometric quantification was carried out in multiple reaction monitoring (MRM) mode, monitoring the m/z transitions 363.1→245.1 for revaprazan and 531.2→489.2 for ketoconazole (internal standard, IS) in positive ion mode. The linear calibration curves covered a concentration range of 2-1,000μgL−1. The intra- and interday precisions (percentage relative standard deviation, RSD%) for revaprazan at three quality control levels were all <5%, and the accuracies were between 90% and 110%. The method has been successfully applied to a pharmacokinetic study involving 12 Chinese volunteers, and the main pharmacokinetic parameters of revaprazan in Chinese population are reported for the first time. Springer-Verlag, 2011 Column liquid chromatography nationallicence LC-ESI-MS-MS nationallicence Human plasma nationallicence Pharmacokinetics nationallicence Revaprazan nationallicence Yu Peng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Cheng Hang Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut Guo Xin Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut Liu Zhi Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut Li Xiao School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Cheng Zeneng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 667-672 0009-5893 73:7-8<667 2011 73 10337 https://doi.org/10.1007/s10337-011-1951-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1951-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Peng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Cheng Hang Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut NATIONALLICENCE 700 1- Guo Xin Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut NATIONALLICENCE 700 1- Liu Zhi Hunan Tiger-Xiangya R.&D. Company, 410013, Changsha, China aut NATIONALLICENCE 700 1- Li Xiao School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Cheng Zeneng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 667-672 0009-5893 73:7-8<667 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer