caa a22 4500 445801638 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10337-011-1930-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1930-9 HILIC-MS-MS for the Quantification of Pidotimod in Human Plasma [Elektronische Daten] [H. Chen, M. Shen, L. Chen] A method for fast, sensitive, and specific hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS) was developed for the first time to determine the level of pidotimod in human plasma. With rosiglitazone as internal standard, analysis was carried out on a HILIC column (150mm×2.1mm, 3.5μm) using a mobile phase consisting of methanol:0.2% formic acid (60:40, v/v). Detection was carried out by tandem mass spectrometry using electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 11.2-1.12×104ngmL−1 for pidotimod, with a lower limit of quantification of 11.2ngmL−1. The intra- and inter-day precision values were high, with standard deviations lower than 15%, and the accuracy, in terms of relative error, ranged from −10.5 to 9.4% at all quality control (QC) levels. Springer-Verlag, 2011 HILIC-MS/MS nationallicence ESI nationallicence Human plasma nationallicence Pidotimod nationallicence Rosiglitazone nationallicence Chen H. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Science, 510640, Guangzhou, China aut Shen M. Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, 510515, Guangzhou, China aut Chen L. Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, 510515, Guangzhou, China aut Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 767-773 0009-5893 73:7-8<767 2011 73 10337 https://doi.org/10.1007/s10337-011-1930-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1930-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen H. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Science, 510640, Guangzhou, China aut NATIONALLICENCE 700 1- Shen M. Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 700 1- Chen L. Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, 510515, Guangzhou, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/7-8(2011-04-01), 767-773 0009-5893 73:7-8<767 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer