caa a22 4500 445801670 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-010-1877-2 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1877-2 Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide [Elektronische Daten] [Yun Wei, Yali Gao, Qianqian Xie, Guoliang Zhang, Weidong Fu] Preparative counter-current chromatography (CCC) was successfully used for isolation and purification of chlorogenic acid from Flaveria bidentis (L.) Kuntze with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 50:1:50, v/v. Using a preparative unit of the CCC centrifuge, about 800mg of the crude extract was separated, yielding 3.2mg of chlorogenic acid at a purity of 92.0%. The blood pressure lowering and antivirus chlorogenic acid (C16H18O9) was intercalated into magnesium-aluminum-layered double hydroxides, which was used as host materials for drug-LDH host-guest supermolecular structures by anion exchange under a nitrogen atmosphere. Chlorogenic acid-LDH is a functional and effective drug. The product chlorogenic acid-LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA) and scanning electron micrographs (SEM). The X-ray diffraction patterns of NO3 − form of LDH and chlorogenic acid-LDH were compared, and the basal d spacing value of NO3 −-LDH layer was 8.75Å (2θ=10.100°); however, the basal reflection (003) of chlorogenic acid-LDH shifts to lower 2θ (for 003 reflection: 2θ=5.119°) that is expanded to 17.25Å, indicating the intercalation of chlorogenic acid into the interlayer of Mg-Al-LDH. Thermogravimetric analysis showed that chlorogenic acid stability had improved, and scanning electron micrographs showed that the morphology of the chlorogenic acid-LDH was irregular masses of distinctly thicker flakes, which was similar to the morphology of NO3 − form of LDH. Springer-Verlag, 2011 Counter-current chromatography nationallicence Layered double hydroxides nationallicence Chlorogenic acid nationallicence Flaveria bidentis (L.) Kuntze nationallicence Wei Yun State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Gao Yali State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Xie Qianqian State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Zhang Guoliang Institute of Environment and Sustainable Development in Agriculture, The Chinese Academy of Agricultural Sciences, 12 South Street Zhongguancun, 100081, Beijing, People's Republic of China aut Fu Weidong Institute of Environment and Sustainable Development in Agriculture, The Chinese Academy of Agricultural Sciences, 12 South Street Zhongguancun, 100081, Beijing, People's Republic of China aut Chromatographia Springer-Verlag 73(2011-06-01), 97-102 0009-5893 73<97 2011 73 10337 https://doi.org/10.1007/s10337-010-1877-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1877-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Yun State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yali State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xie Qianqian State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Guoliang Institute of Environment and Sustainable Development in Agriculture, The Chinese Academy of Agricultural Sciences, 12 South Street Zhongguancun, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Fu Weidong Institute of Environment and Sustainable Development in Agriculture, The Chinese Academy of Agricultural Sciences, 12 South Street Zhongguancun, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 97-102 0009-5893 73<97 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer