caa a22 4500 445801727 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-1916-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1916-7 Analysis of Phenolic Compounds in Lager Beers of Different Origin: A Contribution to Potential Determination of the Authenticity of Czech Beer [Elektronische Daten] [Ivana Marova, Katerina Parilova, Zdenek Friedl, Stanislav Obruca, Katerina Duronova] Beer contains a wide range of polyphenolic compounds originating mainly from malt and hops. In this work newly modified on-line coupled HPLC-photodiode-array (PDA)-MS methods were used for analysis of characteristic phenolic compounds in several Czech lager beers, in comparison with some foreign lager beers. After optimization of column type, elution mode, and gradient steepness, chromatography was performed with a Restek Ultra Aqueous, C18 (5μm, 250mm×4.6mm) column at 30°C and gradient elution using an optimized linear gradient of aqueous acetonitrile acidified with 1% acetic acid, at a flow rate 0.4mLmin−1. In total, 49 compounds were identified. Eleven individual compounds, predominantly malt phenolics (gallic acid, (−)-catechin, epicatechin, ferulic acid, chlorogenic acid, morin, rutin, quercetin, caempherol, naringenin, and luteolin) were quantified by use of two detection techniques: MS with electrospray ionization and UV detection. Compared with foreign beers, Czech beers contained higher levels of most of the phenolic compounds; specific distributions of individual compounds were also observed. Experimental PDA results for individual polyphenols were evaluated statistically by modified cluster analysis. Because of very tight covariance of the data a new procedure was devised for correlation analysis. The set of beers analyzed can be divided into four clusters closely related to the origin of the and the technology used. It seems that some of the flavonoids have potential use in beer authenticity analysis. Springer-Verlag, 2011 HPLC-PDA-MS nationallicence Beer nationallicence Lager nationallicence Czech beer nationallicence Phenolics nationallicence Flavonoids nationallicence Marova Ivana Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut Parilova Katerina Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut Friedl Zdenek Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut Obruca Stanislav Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut Duronova Katerina Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut Chromatographia Springer-Verlag 73(2011-06-01), 83-95 0009-5893 73<83 2011 73 10337 https://doi.org/10.1007/s10337-011-1916-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1916-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marova Ivana Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Parilova Katerina Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Friedl Zdenek Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Obruca Stanislav Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Duronova Katerina Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 83-95 0009-5893 73<83 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer