caa a22 4500 445801735 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-1959-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1959-9 LC-ESI-MS-MS Method for Monitoring Dopamine, Serotonin and Their Metabolites in Brain Tissue [Elektronische Daten] [Věra Najmanová, Lukáš Rambousek, Kamila Syslová, Věra Bubeníková, Romana Šlamberová, Karel Valeš, Petr Kačer] A rapid and precise LC-ESI-MS-MS method for the parallel identification and quantification of dopamine, serotonin and their metabolites (homovanillic acid, 3-methoxytyramine, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolacetic acid) from rat brain tissue without any pre-analysis adjustment of the sample such as pre-concentration or derivatization has been developed. In particular, the reaction-monitoring mode was selected for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. Alternation the ionization polarity in the course of mass spectrometry detection enabled to determine substances susceptible to various ionization modes in only one analysis run. This fact, in combination with an easy pre-treatment step, constitutes the method straightforward and time undemanding. The developed method was characterized with a high precision (≤19.5%, determined as RSD), an acceptable accuracy (≥82.0%, determined as recovery), a low limit of detection (≤0.40ng/100mg brain tissue) and a low limit of quantification (≤0.42ng/100mg brain tissue). The method has been applied in a recent animal study. The levels of the studied neurotransmitters have been determined in the rat brain hippocampus, prefrontal cortex, and striatum in an animal model of schizophrenia induced by an acute dose of a dizocilpine. Springer-Verlag, 2011 LC-ESI-MS-MS nationallicence Dopamine nationallicence Serotonin nationallicence Homovanillic acid nationallicence 3-Methoxytyramine nationallicence 3,4-Dihydroxyphenylacetic acid nationallicence 5-Hydroxyindolacetic acid nationallicence Najmanová Věra Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Rambousek Lukáš Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Syslová Kamila Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Bubeníková Věra Department of Biochemistry and Brain Pathophysiology, Prague Psychiatric Center, Ustavni 91, 181 03, Prague 8, Czech Republic aut Šlamberová Romana Department of Normal Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00, Prague 10, Czech Republic aut Valeš Karel Institute of Physiology, Videnska, 1083 142 20, Prague 4, Czech Republic aut Kačer Petr Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut Chromatographia Springer-Verlag 73(2011-06-01), 143-149 0009-5893 73<143 2011 73 10337 https://doi.org/10.1007/s10337-011-1959-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1959-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Najmanová Věra Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Rambousek Lukáš Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Syslová Kamila Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Bubeníková Věra Department of Biochemistry and Brain Pathophysiology, Prague Psychiatric Center, Ustavni 91, 181 03, Prague 8, Czech Republic aut NATIONALLICENCE 700 1- Šlamberová Romana Department of Normal Pathological and Clinical Physiology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00, Prague 10, Czech Republic aut NATIONALLICENCE 700 1- Valeš Karel Institute of Physiology, Videnska, 1083 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Kačer Petr Institute of Chemical Technology, Technicka 5, 166 28, Prague 6, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 143-149 0009-5893 73<143 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer