caa a22 4500 445801751 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-010-1887-0 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1887-0 HPLC Separation of Fluorescent Products of Lipid Peroxidation in Erythrocytes and Mitochondria [Elektronische Daten] [Joško Ivica, Alice Skoumalová, Eva Topinková, Jiří Wilhelm] Polyunsaturated fatty acids found in membrane phospholipids are readily oxidized by free radicals producing highly reactive aldehydes such as malondialdehyde. These aldehydes react with biological material to form fluorescent lipid peroxidation end products known as lipofuscin-like pigments. We studied fluorescent pigments from beef heart mitochondria incubated with malondialdehyde in vitro and from erythrocytes of patients with Alzheimer's disease to evaluate their potential as markers of oxidative stress and to develop HPLC methods for their qualitative analysis. We used tridimensional fluorescence spectral arrays and synchronous fluorescence spectra in connection with HPLC separation with fluorescence detection. Stable fluorophores were found in both models and were successfully resolved into several distinctive fractions. This creates the basis for further characterization of this relatively less studied group of products of oxidative damage. Springer-Verlag, 2011 Column liquid chromatography nationallicence Lipofuscin-like pigments nationallicence Malondialdehyde nationallicence Mitochondria nationallicence Erythrocytes nationallicence Ivica Joško Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut Skoumalová Alice Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut Topinková Eva Department of Geriatrics, 1st Medical Faculty, Charles University in Prague, Londýnská 15, 120 00, Prague, Czech Republic aut Wilhelm Jiří Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut Chromatographia Springer-Verlag 73(2011-06-01), 67-73 0009-5893 73<67 2011 73 10337 https://doi.org/10.1007/s10337-010-1887-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1887-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ivica Joško Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut NATIONALLICENCE 700 1- Skoumalová Alice Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut NATIONALLICENCE 700 1- Topinková Eva Department of Geriatrics, 1st Medical Faculty, Charles University in Prague, Londýnská 15, 120 00, Prague, Czech Republic aut NATIONALLICENCE 700 1- Wilhelm Jiří Department of Medical Chemistry and Biochemistry, 2nd Medical Faculty, Charles University in Prague, Plzeňská 221/130, 150 06, Prague, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 67-73 0009-5893 73<67 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer