caa a22 4500 44580176X CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-1958-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-1958-x Optimization of Modern Analytical SPME and SPDE Methods for Determination of Trans -2-nonenal in Barley, Malt and Beer [Elektronische Daten] [Zdenek Svoboda, Renata Mikulíková, Sylvie Běláková, Karolína Benešová, Ivana Márová, Zdenek Nesvadba] Trans-2-nonenal is an aldehyde contributing to an unpleasant off-flavor and odor of rancid butter in stored beer. The automated solid-phase microextraction technique (SPME) coupled with gas chromatography (GC) and solid-phase dynamic extraction (SPDE) coupled with gas chromatography were optimized and introduced to determine trans-2-nonenal in barley, malt and beer. Five types of SPME fibers coated with different stationary phases (100μm PDMS, 65μm PDMS/DVB, 85μm CAR/PDMS, 50/30μm DVB/CAR/PDMS, 85μm PA) and two needles (PDMS, PDMS/AC) were compared and tested for their efficiencies in the headspace (HS) SPME and SPDE determination of trans-2-nonenal in barley, malt and beer. The highest extraction efficiency of HS-SPME was achieved with the PDMS/DVB fiber, and addition of 1.5g of NaCl, extraction time was 20min at 60°C. The highest extraction efficiency of HS-SPDE was obtained with the PDMS needle, 15 extraction strokes at 60°C and addition of 1.5g of NaCl. Trans-2-nonenal was identified with the method of HS-SPME coupled gas chromatography-mass spectrometry (GC-MS); the samples were analyzed using the HS-SPME-GC-coupled gas chromatography-flame ionization detector (GC-FID) technique. Springer-Verlag, 2011 Gas chromatography nationallicence Mass spectrometry nationallicence Solid-phase microextraction nationallicence Solid-phase dynamic extraction nationallicence Barley nationallicence Malt and beer nationallicence Trans -2-nonenal nationallicence Svoboda Zdenek Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut Mikulíková Renata Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut Běláková Sylvie Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut Benešová Karolína Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut Márová Ivana Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00, Brno, Czech Republic aut Nesvadba Zdenek Agrotest Fyto, Havlíčkova 2787/121, 768 01, Kroměříž, Czech Republic aut Chromatographia Springer-Verlag 73(2011-06-01), 157-161 0009-5893 73<157 2011 73 10337 https://doi.org/10.1007/s10337-011-1958-x text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1958-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Svoboda Zdenek Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Mikulíková Renata Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Běláková Sylvie Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Benešová Karolína Research Institute of Brewing and Malting, Malting Institute, Mostecká 7, 614 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Márová Ivana Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Nesvadba Zdenek Agrotest Fyto, Havlíčkova 2787/121, 768 01, Kroměříž, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 157-161 0009-5893 73<157 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer