caa a22 4500 445801786 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-010-1878-1 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1878-1 Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol [Elektronische Daten] [Eli Wilson, Saïd Ennahar, Minjie Zhao, Martine Bergaentzle, Eric Marchioni, Françoise Bindler] Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7μgg−1 fresh weight, respectively. The Author(s), 2011 Column liquid chromatography nationallicence UV-detection nationallicence Mass spectrometry nationallicence Isothiocyanates nationallicence Solubility nationallicence Mercaptoethanol nationallicence Wilson Eli Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Ennahar Saïd Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Zhao Minjie Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Bergaentzle Martine Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Marchioni Eric Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Bindler Françoise Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut Chromatographia Springer-Verlag 73(2011-06-01), 137-142 0009-5893 73<137 2011 73 10337 https://doi.org/10.1007/s10337-010-1878-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1878-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wilson Eli Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 700 1- Ennahar Saïd Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 700 1- Zhao Minjie Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 700 1- Bergaentzle Martine Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 700 1- Marchioni Eric Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 700 1- Bindler Françoise Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400, Illkirch, France aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 137-142 0009-5893 73<137 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer