caa a22 4500 445801794 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-1938-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1938-1 HPLC Method for Monitoring of Degradation Products in Picoplatin Stability Study [Elektronische Daten] [Jitka Housková, Michaela Hrdá, Kamila Syslová, Petr Novotný, Petr Kačer] An analytical method combining high-performance liquid chromatography (HPLC) with UV detection was developed for an easy and rapid assay determination of the anticancer drug picoplatin (=cis-[Pt(NH3)(2-methylpyridine)Cl2]) and its degradation products. An ion exchanger was used as the stationary phase with an aqueous solution of NaH2PO4 with pH adjusted to 3.0 with H3PO4 as the mobile phase. The calibration curve was linear within the concentration range of 0.10-0.50gL−1 (R 2≥0.998). The limit of detection was 0.05gL−1 and limit of quantification was 0.09gL−1. The developed method was characterized with a high precision (≤6.0%, determined as RSD), an acceptable accuracy (the values of recovery were from intervals 98-103%). The developed method was used for assessing the stability of picoplatin during a stability study. Springer-Verlag, 2011 Column liquid chromatography nationallicence Stress stability study nationallicence Anticancer drug nationallicence Picoplatin nationallicence Housková Jitka Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut Hrdá Michaela Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut Syslová Kamila Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut Novotný Petr Essence Line, Plzenska 130/221, 150 00, Prague, Czech Republic aut Kačer Petr Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut Chromatographia Springer-Verlag 73(2011-06-01), 131-135 0009-5893 73<131 2011 73 10337 https://doi.org/10.1007/s10337-011-1938-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1938-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Housková Jitka Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Hrdá Michaela Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Syslová Kamila Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Novotný Petr Essence Line, Plzenska 130/221, 150 00, Prague, Czech Republic aut NATIONALLICENCE 700 1- Kačer Petr Department of Organic Technology, Faculty of Chemical Technology, Institute of Chemical Technology, Technicka 5, 166 28, Prague, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73(2011-06-01), 131-135 0009-5893 73<131 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer