caa a22 4500 445801867 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1884-3 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1884-3 Optimal Template Removal from Molecularly Imprinted Polymers by Pressurized Hot Water Extraction [Elektronische Daten] [Bareki Batlokwa, Janes Mokgadi, Tebello Nyokong, Nelson Torto] An optimal extraction method for the removal of templates from molecularly imprinted polymers (MIPs) is presented. The extraction method is based on pressurized hot water extraction (PHWE). PHWE was evaluated by application to three distinctly colored MIPs for chlorophyll (green), quercetin (yellow) and phthalocynine (dark blue) with subsequent monitoring of template removal and template bleeding by an ultraviolet spectrophotometer. The templates were washed-off and the extraction efficiency (EE) was compared to that of soxhlet and ultrasonic extraction methods. PHWE employed hot water at an optimal temperature of 220°C, pressure of 50 bars and flow rate of 2mLmin−1 to thoroughly wash-off the respective templates from their MIPs. The EE evaluated for PHWE was over 99.6% for all the MIPs with no subsequent or minimal template bleeding (<0.01%). The washing procedure was simple and relatively fast as it was achieved in 70min at the most. At 95% confidence level (n=3), soxhlet and ultrasonic recorded EE that was not significantly different (<94.5% in all cases) from that of PHWE (>99.6% in all cases). Soxhlet and ultrasonic had washing procedures that were slower (over 18h) and employed large quantities (400mL) of organic solvents modified with acids. The percentage relative standard deviations (%RSD) for the EE and recovery results were less than 2.3% in all cases indicating the high reproducibility of the method. Overall, the three methods performed comparably in extracting templates. PHWE seems to be the method of choice as it employed water which poses no environmental threat. Springer-Verlag, 2011 Pressurized hot water extraction nationallicence Green method of extraction nationallicence Molecularly imprinted polymers nationallicence Template removal nationallicence Batlokwa Bareki Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut Mokgadi Janes Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut Nyokong Tebello Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut Torto Nelson Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 589-593 0009-5893 73:5-6<589 2011 73 10337 https://doi.org/10.1007/s10337-010-1884-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1884-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Batlokwa Bareki Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Mokgadi Janes Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Nyokong Tebello Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Torto Nelson Department of Chemistry, Rhodes University, P.O. Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 589-593 0009-5893 73:5-6<589 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer