caa a22 4500 445801883 CHVBK 20180317145143.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1905-2 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1905-2 CE Method with Partial Filling Techniques for Screening of Xanthine Oxidase Inhibitor in Traditional Chinese Medicine [Elektronische Daten] [Lichun Zhang, Kun Hu, Xiangtang Li, Shulin Zhao] A combination of electrophoretically mediated microanalysis (EMMA) methodology with a partial filling technique was developed for screening of xanthine oxidase (XOD) inhibitors in substances used in traditional Chinese medicine (TCM). In order to achieve sufficient separation, a micellar electrokinetic chromatography (MEKC) method was employed for the separation. The enzyme activity was determined by the quantification of the peak area of the product, uric acid (UA), at 295nm. Enzyme inhibition can be read out directly from the reduced peak area of UA in comparison to a reference electropherogram obtained in the absence of any inhibitor. The method was validated using a commercially available XOD inhibitor, 4-aminopyrazolo[3,4-d]pyrimidine, and the IC50 value was determined to be 29.90±0.26μM. Fifteen natural extracts from TCM were screened, and Cortex Phellodendri (the dried bark of Phellodendron chinense) extract was found to be positive for XOD inhibition. Springer-Verlag, 2011 Capillary electrophoresis nationallicence Inhibitor screening nationallicence Partial filling techniques nationallicence Xanthine oxidase nationallicence Zhang Lichun The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut Hu Kun The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut Li Xiangtang The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut Zhao Shulin The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 583-587 0009-5893 73:5-6<583 2011 73 10337 https://doi.org/10.1007/s10337-010-1905-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1905-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Lichun The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Hu Kun The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Li Xiangtang The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 700 1- Zhao Shulin The Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 583-587 0009-5893 73:5-6<583 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer