caa a22 4500 445801891 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1929-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1929-2 Determination of Five Polyphenols by HPLC/DAD and Discrimination of Apple Varieties [Elektronische Daten] [Ning-Ning Chen, Shan-Cang Zhao, Li-Gang Deng, Chang-Ying Guo, Jiang-Sheng Mao, Hong Zheng, Guo-Sheng Yang, Xiao Lu, Hassan Aboul-Enein] The objective of this study was to set up a method to detect five compounds in fresh smashed apples by HPLC/DAD simultaneously. Different methods have been tested to control browning and ascorbic acid with ultrasonication was adopted. Methanol-water-acetic acid (30:69:1, v/v) containing 2.0g of ascorbic acidL−1 was chosen as the extract solvent. The method effectively simplified the sample treatment compared with the traditional ways. And primarily, the results were used to identify between different varieties. The chromatographic separation was performed on an Atlantis C18 (250mm×4.5mm, particle size 5μm) with a gradient elution program using a mixture of acetonitrile and 2% aqueous acetic acid (v/v) as mobile phase within 20min at 270nm wavelength. The variation of the content of five compounds was gallic acid (ND ~1.81μgg−1), protocatechuic acid (ND ~1.79μgg−1), chlorogenic acid (13.81-189.4μgg−1), caffeic acid (6.82-45.02μgg−1) and rutin (0.96-18.55μgg−1). The results could successfully be used to discriminate between different apple varieties (Gala, Fuji, Delicious, 8th Apple US, Golden Apple, Green Apple and Red Rose); chlorogenic acid and rutin being the polyphenols that contribute most to the differentiation. Springer-Verlag, 2011 Column liquid chromatography nationallicence HPLC/DAD nationallicence Polyphenols nationallicence Discrimination nationallicence Apple varieties nationallicence Chen Ning-Ning School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut Zhao Shan-Cang Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut Deng Li-Gang Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut Guo Chang-Ying Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut Mao Jiang-Sheng Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut Zheng Hong School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut Yang Guo-Sheng School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut Lu Xiao Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut Aboul-Enein Hassan Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, 12311, Cairo, Egypt aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 595-598 0009-5893 73:5-6<595 2011 73 10337 https://doi.org/10.1007/s10337-011-1929-2 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1929-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Ning-Ning School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Shan-Cang Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Deng Li-Gang Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Guo Chang-Ying Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Mao Jiang-Sheng Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Hong School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Yang Guo-Sheng School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Lu Xiao Central Laboratory of Shandong Academy of Agricultural Sciences, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Aboul-Enein Hassan Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, 12311, Cairo, Egypt aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 595-598 0009-5893 73:5-6<595 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer