caa a22 4500 445801921 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1911-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-1911-z Micellar Electrokinetic Chromatography Using a Cationic Surfactant for Rapid Separation and Determination of Bisbenzylisoquinoline Alkaloids from Embryo of the Seed of Nelumbo nucifera Gaertn [Elektronische Daten] [Jinhua Zhu, Huige Zhang, Shengda Qi, Xingguo Chen, Zhide Hu] Liensinine (LIE), isoliensinine (ISO) and neferine (NEF) from embryo of the seed of Nelumbo nucifera Gaertn were first separated and quantitatively analyzed by micellar electrokinetic chromatography with internal standard (IS) using a cationic surfactant, tetradecyltrimethyl ammonium bromide (TTAB). Complete separation of the analytes was optimally achieved within 15min using 40mM sodium dihydrogenphosphate (pH 6.40) containing 10mM TTAB and 15% (v/v) methanol as buffer. Tetrahydropalmatine was used as IS to improve the repeatability and linearity relativity. The IS method resulted in excellent linearity, with correlation coefficients of regression equations of 0.9997, 0.9997 and 0.9992 for LIE, ISO and NEF, respectively. Finally, two sample extraction methods were used to investigate the contents of different parts of the embryos. Springer-Verlag, 2011 Micellar electrokinetic chromatography nationallicence Cationic surfactant nationallicence Bisbenzylisoquinoline alkaloids nationallicence Zhu Jinhua Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Zhang Huige Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Qi Shengda Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Chen Xingguo Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Hu Zhide Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 535-540 0009-5893 73:5-6<535 2011 73 10337 https://doi.org/10.1007/s10337-011-1911-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1911-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Jinhua Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Huige Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Qi Shengda Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xingguo Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Zhide Department of Chemistry, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 535-540 0009-5893 73:5-6<535 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer