caa a22 4500 445801964 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1908-z doi (NATIONALLICENCE)springer-10.1007/s10337-010-1908-z LC Tissue Distribution Study of Paeonol in Rats after Oral Administration [Elektronische Daten] [Hua Li, Siwang Wang, Qian Yang, Yanhua Xie, Wei Cao, Bangle Zhang, Jianbo Wang, Jiepin Wang, Meng Wang] Paeonol, an important constituent of the traditional Chinese medicine Cortex Moutan, has a variety of bioactivity. A simple and sensitive HPLC-UV method has been developed for analysis of paeonol in different rat tissue (heart, liver, spleen, lung, kidney, and brain). Bio-samples were prepared by simple protein precipitation, and separation of paeonol was achieved on a C18 column with methanol-2% glacial acetic acid solution 70:30 (v/v) as mobile phase at a flow rate of 1.0mLmin−1. UV detection was at 274nm and the column temperature was 30°C. Linearity was good between 0.025 and 5.0μgmL−1 (r 2≥0.9990) for tissue samples. Inter-day and intra-day accuracy (as relative error, RE) and precision (as relative standard deviation, RSD) were <5.94 and 6.05%, respectively. The limit of detection was 0.025μgmL−1 and extraction recovery for all samples was ≥85.86%. The method was successfully applied to a tissue-distribution study after oral administration of 40mgkg−1 paeonol to healthy Sprague-Dawley rats. The study showed that paeonol was quickly distributed and eliminated after oral administration; liver and kidney were the major distribution tissues of paeonol in rats, and paeonol quickly passed through the blood-brain barrier. It was also found there was no long-term accumulation of paeonol in rat tissues. Springer-Verlag, 2011 Column liquid chromatography nationallicence Tissue distribution nationallicence Paeonol nationallicence Cortex Moutan nationallicence Li Hua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Wang Siwang Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Yang Qian Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Xie Yanhua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Cao Wei Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Zhang Bangle Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Wang Jianbo Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Wang Jiepin Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Wang Meng Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 495-500 0009-5893 73:5-6<495 2011 73 10337 https://doi.org/10.1007/s10337-010-1908-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1908-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Wang Siwang Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Yang Qian Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Xie Yanhua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Cao Wei Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Bangle Department of Pharmaceutics, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jianbo Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jiepin Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Wang Meng Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 145 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 495-500 0009-5893 73:5-6<495 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer