caa a22 4500 445801980 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1913-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-1913-x Improved Method for Stratum Corneum Lipid Analysis by Automated Multiple Development HPTLC [Elektronische Daten] [Annett Opitz, Michaela Wirtz, David Melchior, Annette Mehling, Hans-Willi Kling, Reinhard Neubert] An improved high-performance thin-layer chromatographic (HPTLC) method has been developed for quantitative analysis of stratum corneum lipids. When used for quantification of in-vivo-extracted skin lipids, the optimized method resulted in improved separation of cholesterol, cholesteryl esters, free fatty acids, and squalene. Variation in repeatability for cholesterol, cholesteryl esters and free fatty acids was under ±5%. The extraction recovery was 91.4±1.2% (mean±CV). Coefficients of variation ranged from 0.8 to 3.8%. The limits of detection S/N 3:1 and of quantification S/N 5:1 under the given conditions were: cholesteryl sulfate 12.5/25ng, ceramide [AP]D-compound 50/250ng, ceramide [NP] 50/125ng, ceramide [NS] 125/250ng, cholesterol 50/125ng, palmitic acid 50/250ng, and cholesteryl oleate 50/125ng. Springer-Verlag, 2011 HPTLC nationallicence Automated multiple development nationallicence Stratum corneum lipids nationallicence Ceramides nationallicence Fatty acids nationallicence Cholesterol nationallicence Opitz Annett Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany aut Wirtz Michaela Cognis GmbH, Düsseldorf, Germany aut Melchior David Cognis GmbH, Düsseldorf, Germany aut Mehling Annette Cognis GmbH, Düsseldorf, Germany aut Kling Hans-Willi Cognis GmbH, Düsseldorf, Germany aut Neubert Reinhard Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 559-565 0009-5893 73:5-6<559 2011 73 10337 https://doi.org/10.1007/s10337-011-1913-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1913-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Opitz Annett Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany aut NATIONALLICENCE 700 1- Wirtz Michaela Cognis GmbH, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Melchior David Cognis GmbH, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Mehling Annette Cognis GmbH, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Kling Hans-Willi Cognis GmbH, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Neubert Reinhard Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 559-565 0009-5893 73:5-6<559 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer