caa a22 4500 445801999 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1920-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-1920-y Rapid and Selective LC-MS-MS Method for the Determination of cyclo - trans -4- l -Hydroxyprolyl- l -serine (JBP485) in Rat Plasma [Elektronische Daten] [Changyuan Wang, Jian Cang, Qi Liu, Qiang Meng, Huijun Sun, Ying Gao, Decheng Lv, Taiichi Kaku, Kexin Liu] A rapid and selective liquid chromatographic/tandem mass spectrometric method for the determination of JBP485 was developed and validated. Following protein precipitation, the analyte and internal standard (JBP923) were separated from human plasma using an isocratic mobile phase on an Elite Kromasil C18 column. An API 3200 tandem mass spectrometer equipped with a Turbo ionSpray ionization source was used as the detector and operated in the positive ion mode. Multiple reaction monitoring using the precursor to product ion combinations of m/z 201.2→86.2 and m/z 219.2→86.2 was performed to quantify JBP485 and JBP923, respectively. The method was linear in the concentration range of 0.10-50.00μgmL−1 using 100μL of plasma. The lower limit of quantification was 0.10μgmL−1. The intra- and inter-day relative standard deviations over the entire concentration range were less than 6.65%. Accuracy determined at three concentrations (0.25, 4.00 and 25.00μgmL−1 for JBP485) ranged from −0.78 to 2.74% in terms of relative error. Each plasma sample was chromatographed within 2.0min. The method was successfully applied to characterize the pharmacokinetic profiles of JBP485 in rats after an intravenous injection of 6.25mgkg−1 JBP485. Springer-Verlag, 2011 LC-MS-MS nationallicence Pharmacokinetics nationallicence JBP485 nationallicence JBP923 nationallicence Wang Changyuan Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Cang Jian Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Liu Qi Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Meng Qiang Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Sun Huijun Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Gao Ying Provincial Key Laboratory of Molecular Biology, 116044, Dalian, Liaoning, China aut Lv Decheng Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University, 116044, Dalian, Liaoning, China aut Kaku Taiichi Japan Bioproducts Industry Co Ltd., Tomigaya, Shibuya-ku, Tokyo, Japan aut Liu Kexin Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 481-486 0009-5893 73:5-6<481 2011 73 10337 https://doi.org/10.1007/s10337-011-1920-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1920-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Changyuan Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Cang Jian Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Liu Qi Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Meng Qiang Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Sun Huijun Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Gao Ying Provincial Key Laboratory of Molecular Biology, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Lv Decheng Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 700 1- Kaku Taiichi Japan Bioproducts Industry Co Ltd., Tomigaya, Shibuya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Liu Kexin Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, 9 West Section, Lvshun South Road, Lvshunkou District, 116044, Dalian, Liaoning, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 481-486 0009-5893 73:5-6<481 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer