caa a22 4500 445802014 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1903-4 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1903-4 Simultaneous LC-MS Analysis of Paclitaxel and Retinoic Acid in Plasma and Tissues from Tumor-Bearing Mice [Elektronische Daten] [Lin Hou, Jing Yao, Jianping Zhou] A novel method has been developed for simultaneous analysis of paclitaxel (PTX) and retinoic acid (ATRA) in mice plasma and tissue homogenates. The analyte was isolated by liquid-liquid partitioning, to minimize analyte degradation, and fractions were analyzed by liquid chromatography-mass spectrometry (LC-MS). Liquid-liquid extraction with tert-butyl methyl ether was used for sample preparation and docetaxel was used as internal standard (IS). Separation of PTX, ATRA, and the IS was performed on a C18 column with gradient elution and detection by use of a single-quadrupole mass spectrometer in selected-ion-monitoring mode. The method had high extraction recovery (>85%) and accuracy (>90%), with intra-day and inter-day precision <15%, and was rugged with good linearity. The limits of quantitation were determined to be 2 and 4ngmL−1 for PTX and ATRA, respectively. Freeze-thaw stability, short-term stability, long-term stability, and sample stability in the autosampler tray were examined; this indicated freezing and thawing during bio-sample preparation should be avoided, but no other stability-related problems occurred during sample storage, extraction, and chromatography of PTX and ATRA in plasma and tissue samples. The assay was successfully used for simultaneous analysis of PTX and ATRA in mice plasma and different tissues to support pharmacokinetic and in-vivo distribution studies of the two drugs. Springer-Verlag, 2011 Liquid chromatography-mass spectrometry nationallicence Simultaneous analysis nationallicence Paclitaxel nationallicence Retinoic acid nationallicence Pharmacokinetics nationallicence In-vivo distribution nationallicence Hou Lin Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Yao Jing Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Zhou Jianping Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 471-480 0009-5893 73:5-6<471 2011 73 10337 https://doi.org/10.1007/s10337-010-1903-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1903-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hou Lin Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Yao Jing Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Zhou Jianping Department of Pharmaceutics, China Pharmaceutical University, 24 Tongjiaxiang, 210009, Nanjing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 471-480 0009-5893 73:5-6<471 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer