caa a22 4500 445802022 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1907-0 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1907-0 Qualitative and Quantitative Determination of Oligonucleotides by Non-Gel Capillary Electrophoresis [Elektronische Daten] [Wujie Li, Yinling Ma, Zhu Gan, Xiaomei Ling, Zhenjun Yang] A novel simple non-gel capillary electrophoresis (NGCE) method was developed for the qualitative and quantitative determination of oligonucleotides (ODNs). ODNs were synthesized in a DNA synthesizer and separated by NGCE under optimized conditions as follows: 25mmolL−1 Tris-boric-EDTA buffer (pH 8.0) with 7mmolL−1 urea in the presence of 20% (w/v) polyethylene glycol (PEG) 35000 at 30°C and −20kV. Single-base differences, in which there are differences in the base quantity and the single-base structure with the same base quantity, can be identified by the proposed method, and the quantitative determination was carried out. The obtained regression equation revealed a good linear relationship between the peak area ratio and the ODNs concentration. The correlation coefficient was 0.9986 and the relative standard deviation (RSD) of the precision was <3.6% (intro-day) and <6.2% (inter-day), respectively. The recoveries of three concentrations (high, middle, and low) were between 99.6% and 107.6%. The established method is sensitive, simple, economical, and can be used in gene mutation detection, pharmacokinetics study of nucleic acid drugs, and so on. Springer-Verlag, 2011 Non-gel capillary electrophoresis nationallicence Polyethylene glycol (PEG) 35000 nationallicence Oligonucleotide nationallicence Li Wujie Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut Ma Yinling Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut Gan Zhu State Key Laboratory of Natural and Biomimetic Drugs, Peking University, 100191, Beijing, China aut Ling Xiaomei Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut Yang Zhenjun State Key Laboratory of Natural and Biomimetic Drugs, Peking University, 100191, Beijing, China aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 579-582 0009-5893 73:5-6<579 2011 73 10337 https://doi.org/10.1007/s10337-010-1907-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1907-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Wujie Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Ma Yinling Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Gan Zhu State Key Laboratory of Natural and Biomimetic Drugs, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Ling Xiaomei Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Yang Zhenjun State Key Laboratory of Natural and Biomimetic Drugs, Peking University, 100191, Beijing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 579-582 0009-5893 73:5-6<579 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer