caa a22 4500 445802030 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1923-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1923-8 Determination of Propylene Glycol in Low Volume Plasma and Urine Samples of Neonates by LC with Photodiode Array Detection [Elektronische Daten] [Aida Kulo, Karel Allegaert, Jan de Hoon, Rene Verbesselt] In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25-50mgL−1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15-46mgL−1) and urine samples (20-175mgL−1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates. Springer-Verlag, 2011 Column liquid chromatography nationallicence Propylene glycol nationallicence Neonate nationallicence Low volume samples nationallicence Developmental toxicology nationallicence Kulo Aida Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut Allegaert Karel Neonatal Intensive Care Unit, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut de Hoon Jan Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut Verbesselt Rene Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 463-470 0009-5893 73:5-6<463 2011 73 10337 https://doi.org/10.1007/s10337-011-1923-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1923-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kulo Aida Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Allegaert Karel Neonatal Intensive Care Unit, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- de Hoon Jan Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Verbesselt Rene Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 463-470 0009-5893 73:5-6<463 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer