caa a22 4500 445802057 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1901-6 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1901-6 Multivariate Optimization and Validation of a CE method for Simultaneous Analysis of Dorzolamide Hydrochloride and Timolol Maleate in Ophthalmic Solution [Elektronische Daten] [Ismail Palabiyik, Mehmet Caglayan, Feyyaz Onur] A fast, accurate, precise, and sensitive capillary electrophoretic method has been developed for simultaneous analysis of dorzolamide hydrochloride and timolol maleate in eye drops. Response-surface methodology with a central composite design was used for optimization of buffer pH, applied potential, and temperature. Capillary electrophoresis at 25°C with NaH2PO4 buffer (pH6.00, 0.025M), and an applied potential of 20kV was found to be suitable, and under these optimum conditions, dorzolamide hydrochloride and timolol maleate were separated in approximately 3min. This method was validated and successfully applied to a pharmaceutical preparation, ophthalmic solution, and the results were compared with those from a spectrophotometric method described in the literature. Springer-Verlag, 2011 Capillary electrophoresis nationallicence Multivariate optimization nationallicence Response-surface methodology nationallicence Dorzolamide hydrochloride nationallicence Timolol maleate nationallicence Palabiyik Ismail Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut Caglayan Mehmet Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut Onur Feyyaz Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 541-548 0009-5893 73:5-6<541 2011 73 10337 https://doi.org/10.1007/s10337-010-1901-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1901-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Palabiyik Ismail Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Caglayan Mehmet Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Onur Feyyaz Department of Analytical Chemistry, Faculty of Pharmacy, University of Ankara, Tandogan, 06100, Ankara, Turkey aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 541-548 0009-5893 73:5-6<541 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer