caa a22 4500 445802065 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-010-1902-5 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1902-5 LC-MS Method for Determination of Amphotericin B in Rabbit Tears and Its Application to Ocular Pharmacokinetic Study [Elektronische Daten] [Rabi Bhatta, Chetan Rathi, Hardik Chandasana, Devendra Kumar, Yashpal Chhonker, Girish Jain] A rapid and sensitive LC-MS-MS method was developed for the quantification of amphotericin B in rabbit tears using natamycin as internal standard. The analyte and internal standard were extracted from the tear sample using a solid phase extraction method. Chromatographic separation was achieved on a Phenomenex Luna 3μm CN column (100×2mm, 3μm) using 3.5mM ammonium acetate (pH 4):methanol (10:90) as mobile phase. The assay was validated with a linear range of 0.1-3.2μgmL−1 for amphotericin B using 10μL of tear sample. The intra- and inter-day assay precision ranged from 2.49 to 4.37 and 2.17 to 5.59%, respectively, and intra- and inter-day assay accuracy was between from 0.27 to 3.32 and −0.51 to 3.72%, respectively. The method was successfully applied to the pharmacokinetic studies of amphotericin B eye drops in rabbit tears. Springer-Verlag, 2011 Column liquid chromatography nationallicence Tandem mass spectrometry nationallicence Ocular pharmacokinetics nationallicence Amphotericin B nationallicence Rabbit tear nationallicence LC-MS-MS nationallicence Antifungal nationallicence Bhatta Rabi Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut Rathi Chetan Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), 229010, Raebareli, India aut Chandasana Hardik Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), 229010, Raebareli, India aut Kumar Devendra Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut Chhonker Yashpal Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut Jain Girish Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 487-493 0009-5893 73:5-6<487 2011 73 10337 https://doi.org/10.1007/s10337-010-1902-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1902-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhatta Rabi Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Rathi Chetan Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), 229010, Raebareli, India aut NATIONALLICENCE 700 1- Chandasana Hardik Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), 229010, Raebareli, India aut NATIONALLICENCE 700 1- Kumar Devendra Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Chhonker Yashpal Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Jain Girish Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, 226001, Lucknow, India aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 487-493 0009-5893 73:5-6<487 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer