caa a22 4500 44580209X CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10337-011-1936-3 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1936-3 Inexpensive Green Method for Diclofenac Assay Utilizing Sequential Injection Chromatography [Elektronische Daten] [Abubakr Idris, Rafea Elgorashe, Fahad Assubaie, Ahmed Alnajjar] Sequential injection chromatography (SIC) is a recent inexpensive miniaturized separation technique. The current manuscript reported the development of a new SIC method for the assay of diclofenac in pharmaceutical formulations. Chemometrics was exploited for the first time to optimize SIC methodology. The 23 full-factorial design was adopted to screen the effect of mobile phase composition, sample volume, and flow rate on retention time and peak height. The optimum chromatographic conditions were: C18 monolithic column (25×4.6mm); mobile phase composition methanol:water (50:50, v/v), sample volume 60μL, flow rate 40μLs−1. The detection was carried out using a miniaturized fiber optic spectrometer set at 284nm. Satisfactorily, separation was achieved in 0.92min with the number of theoretical plates of >180,000 and peak symmetry 1.21. The method enjoyed good precision with relative standard deviation values for intra- and inter-day precision of 1.47 and 2.15%, respectively. The use of large sample volume rendered the method sensitive with the limits of detection and quantification of 0.32 and 1.07μgmL−1, respectively. The remarkable advantages of the method are high rapidity (sample frequency 23 sampleh−1) and reagent saving (total consumed volume 3.0mL) and thus better safety for the environment. Springer-Verlag, 2011 Sequential injection chromatography nationallicence Diclofenac nationallicence Pharmaceutical analysis nationallicence Chemometrics nationallicence Factorial design nationallicence Idris Abubakr Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut Elgorashe Rafea Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut Assubaie Fahad Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut Alnajjar Ahmed Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut Chromatographia Springer-Verlag 73/5-6(2011-03-01), 431-437 0009-5893 73:5-6<431 2011 73 10337 https://doi.org/10.1007/s10337-011-1936-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1936-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Idris Abubakr Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut NATIONALLICENCE 700 1- Elgorashe Rafea Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut NATIONALLICENCE 700 1- Assubaie Fahad Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut NATIONALLICENCE 700 1- Alnajjar Ahmed Department of Chemistry, College of Science, King Faisal University, PO Box 400, 31982, Hofuf, Saudi Arabia aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/5-6(2011-03-01), 431-437 0009-5893 73:5-6<431 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer