caa a22 4500 445802138 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1827-z doi (NATIONALLICENCE)springer-10.1007/s10337-010-1827-z LC-MS-MS Determination of Troxerutin in Plasma and Its Application to a Pharmacokinetic Study [Elektronische Daten] [Xiangyong Cui, Mengliang Zhang, Xin Guan, Lei Yin, Yantong Sun, J. Fawcett, Jingkai Gu] A highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of troxerutin in human plasma using tramadol as internal standard (IS) has been developed and validated. Sample preparation involved liquid-liquid extraction with ethyl acetate-isopropanol (95:5, v/v). The analyte and IS were separated by RP-LC with gradient elution using 10mM ammonium acetate containing 0.1% formic acid and methanol at a flow rate of 0.9mLmin−1. LC-MS-MS in the positive ion mode employed multiple reaction monitoring of the transitions at m/z 743.2→435.3 and m/z 264.1→58.0 for troxerutin and IS, respectively. The assay was linear in the concentration range 0.01-10ngmL−1 with precision and accuracy within assay variability limits as per FDA guidelines. The assay was successfully applied to a pharmacokinetic study involving oral administration of 300mg troxerutin to eight healthy Chinese volunteers. Springer-Verlag, 2010 Column liquid chromatography-tandem mass spectrometry nationallicence Pharmacokinetic study nationallicence Troxerutin in human plasma nationallicence Cui Xiangyong Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Zhang Mengliang Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Guan Xin Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Yin Lei Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Sun Yantong Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Fawcett J. School of Pharmacy, University of Otago, PO Box 56, Dunedin, New Zealand aut Gu Jingkai Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 165-169 0009-5893 73:1-2<165 2011 73 10337 https://doi.org/10.1007/s10337-010-1827-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1827-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Xiangyong Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Mengliang Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Guan Xin Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Yin Lei Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Sun Yantong Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Fawcett J. School of Pharmacy, University of Otago, PO Box 56, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Gu Jingkai Research Center for Drug Metabolism, Jilin University, 130021, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 165-169 0009-5893 73:1-2<165 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer