caa a22 4500 445802146 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1823-3 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1823-3 Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies [Elektronische Daten] [Rui-Hua Dong, Zhong-Ze Fang, Hong-Zhi Gao, Guang-Tao Hao, Guang Liu, Ting-Ting Shan, Ze-Yuan Liu] A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed and validated for the quantification of cepharanthine (CEP) in beagle dog plasma. The chromatographic separation was performed on an Agilent-C18 column and the mobile phase was composed of methanol:water with 10mM ammonium acetate (20:80, v/v). Detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring mode (MRM) to monitor m/z transitions 607→365 for CEP and 285→193 for the internal standard (IS) diazepam. This method exhibited a linear range of 5-2,500ngmL−1. The precision (RSD%) and accuracy (RME%) of the assay were <8.7 and 2.4%, respectively. The limit of quantification was 5ngmL−1 and no significant matrix effect was observed. The validated method has been successfully applied to pharmacokinetic study of CEP in beagle dog. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence Pharmacokinetic study nationallicence Cepharanthine nationallicence Dong Rui-Hua Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Fang Zhong-Ze Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut Gao Hong-Zhi Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Hao Guang-Tao Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Liu Guang Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Shan Ting-Ting Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Liu Ze-Yuan Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 75-81 0009-5893 73:1-2<75 2011 73 10337 https://doi.org/10.1007/s10337-010-1823-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1823-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Rui-Hua Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 700 1- Fang Zhong-Ze Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Gao Hong-Zhi Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 700 1- Hao Guang-Tao Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 700 1- Liu Guang Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 700 1- Shan Ting-Ting Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 700 1- Liu Ze-Yuan Department of Clinical Pharmacology, Affiliated Hospital, Academy of Military Medical Sciences, 100071, Beijing, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 75-81 0009-5893 73:1-2<75 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer