caa a22 4500 445802154 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1826-0 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1826-0 LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification [Elektronische Daten] [Li Kang, Jinmao You, Zhiwei Sun, Chengyan Wang, Zhongyin Ji, Yongping Gao, Yourui Suo, Yulin Li] A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M+H]+ in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10μmolL−1 with coefficients of >0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2pmol of each biogenic amine, were from 20.22 to 109.2fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were <0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory. Springer-Verlag, 2011 Column liquid chromatography nationallicence Pre-column derivatization nationallicence Fluorescence detection nationallicence Mass spectrometry nationallicence Biogenic amines nationallicence Kang Li Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut You Jinmao Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut Sun Zhiwei Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut Wang Chengyan College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut Ji Zhongyin College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut Gao Yongping College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut Suo Yourui Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut Li Yulin Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 43-50 0009-5893 73:1-2<43 2011 73 10337 https://doi.org/10.1007/s10337-010-1826-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1826-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang Li Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut NATIONALLICENCE 700 1- You Jinmao Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut NATIONALLICENCE 700 1- Sun Zhiwei Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut NATIONALLICENCE 700 1- Wang Chengyan College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Ji Zhongyin College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Gao Yongping College of Chemistry Science, Qufu Normal University, 273165, Qufu, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Suo Yourui Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut NATIONALLICENCE 700 1- Li Yulin Northwest Plateau Institute of Biology, Chinese Academy of Sciences, 810001, Xining, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 43-50 0009-5893 73:1-2<43 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer