caa a22 4500 445802162 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1815-3 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1815-3 Determination of Doxapram Hydrochloride in Rabbit Plasma by LC-MS-MS and Its Application [Elektronische Daten] [Guanyang Lin, Jianshe Ma, Lufeng Hu, Xuebao Wang, Jiayin Zhu, Xianqin Wang] A sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS-MS) method for determination of doxapram hydrochloride in rabbit plasma was developed. After addition of urapidil hydrochloride as internal standard (IS), protein precipitation by 10% trichloroacetic acid in methanol (w/v) was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1mm×50mm, 3.5μm) column with acetonitrile-water as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used for quantification using target fragment ions m/z 378.9→291.8 for doxapram hydrochloride and m/z 387.9→204.6 for the IS. Calibration plots were linear over the range of 2-1000ngmL−1 for doxapram hydrochloride in plasma. Lower limit of quantitation (LLOQ) for doxapram hydrochloride was 2ngmL−1. Mean recovery of doxapram hydrochloride from plasma was in the range 83.7-91.5%. RSD of intra-day and inter-day precision were less than 9%, respectively. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of doxapram hydrochloride in rabbit plasma. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence Pharmacokinetic study nationallicence Doxapram hydrochloride nationallicence Lin Guanyang The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, China aut Ma Jianshe Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut Hu Lufeng The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, China aut Wang Xuebao Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Zhu Jiayin Laboratory Animal Center of Wenzhou Medical College, 325035, Wenzhou, China aut Wang Xianqin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 183-187 0009-5893 73:1-2<183 2011 73 10337 https://doi.org/10.1007/s10337-010-1815-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1815-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lin Guanyang The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, China aut NATIONALLICENCE 700 1- Ma Jianshe Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Hu Lufeng The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, China aut NATIONALLICENCE 700 1- Wang Xuebao Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Zhu Jiayin Laboratory Animal Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Wang Xianqin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 183-187 0009-5893 73:1-2<183 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer