caa a22 4500 445802197 CHVBK 20180317145144.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1822-4 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1822-4 RP-LC with Fluorescence Detection of Amino Acids in Rat Brain Synaptosomes [Elektronische Daten] [Xiaoyuan Mao, Yonggang Cao, Dongyu Min, Feng Guo, Ni Xie, Tianbao Chen, Chris Shaw, Jiqun Cai] For the first time, a simple and validated reversed-phase liquid chromatography (RP-LC) with fluorescence detection has been developed for the simultaneous analysis of glutamate (Glu), γ-aminobutyric acid (GABA), glycine (Gly) and taurine (Tau) in Wistar and tremor rats brain synaptosomes. The samples were separated on a C18 analytical column with gradient elution of methanol and 0.1molL−1 potassium acetate at a flow rate of 1mLmin−1. Total run time was approximately 25min. All calibration curves exhibited good linearity (r 2>0.999) within test ranges. The reproducibility was estimated by intra-and inter-day assays and RSD values were less than 2.48%. The recoveries were between 96.32 and 105.21%. The method was successfully applied to the quantification of amino acids in Wistar and tremor rats brain synaptosomes. Through this developed protocol, the levels of Glu in hippocampal and prefrontal cortical synaptosomes of tremor rats were both significantly elevated than those of adult Wistar rats whereas significantly decreased concentrations of GABA and Gly were observed in the hippocampal region of tremor rats without evident difference in the prefrontal cortex between experimental and control groups. In addition, our studies also showed a marked elevation of Tau in tremor rats hippocampal synaptosomes although there was no pronounced difference in the prefrontal cortical region of Wistar and tremor rats. Springer-Verlag, 2011 Column liquid chromatography nationallicence Synaptosome nationallicence Rats nationallicence Amino acids nationallicence Mao Xiaoyuan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Cao Yonggang Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Min Dongyu Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Guo Feng Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Xie Ni Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Chen Tianbao Molecular Therapeutics Research Group, School of Pharmacy, Queen's University, Belfast, Northern Ireland, UK aut Shaw Chris Molecular Therapeutics Research Group, School of Pharmacy, Queen's University, Belfast, Northern Ireland, UK aut Cai Jiqun Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 157-163 0009-5893 73:1-2<157 2011 73 10337 https://doi.org/10.1007/s10337-010-1822-4 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1822-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mao Xiaoyuan Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Cao Yonggang Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Min Dongyu Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Guo Feng Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Xie Ni Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 700 1- Chen Tianbao Molecular Therapeutics Research Group, School of Pharmacy, Queen's University, Belfast, Northern Ireland, UK aut NATIONALLICENCE 700 1- Shaw Chris Molecular Therapeutics Research Group, School of Pharmacy, Queen's University, Belfast, Northern Ireland, UK aut NATIONALLICENCE 700 1- Cai Jiqun Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, 110001, Shenyang, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 157-163 0009-5893 73:1-2<157 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer