caa a22 4500 445802200 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1821-5 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1821-5 Simultaneous Determination of Hydrochlorothiazide and Reserpine in Human Urine by LC with a Simple Pre-Treatment [Elektronische Daten] [Hang Li, Junting He, Qin Liu, Zhaohui Huo, Si Liang, Yong Liang, Yoichiro Ito] A simple, selective and sensitive reversed-phase liquid chromatography method for simultaneous analysis of hydrochlorothiazide and reserpine in human urine was developed and subjected to primary pharmacokinetic study. After a simple protein precipitation using methanol and extraction with ethyl acetate, the analytes were separated on an Elite C18 column at a flow rate of 0.8mLmin−1. The mobile phase was composed of acetonitrile (A) and 0.2% ammonium chloride solution (B) for a gradient elution starting at A:B at 30:70, v/v for 0-6min, linearly raising the percent of A from 30 to 50% (6-9min) and ending at 50:50, v/v (9-25min). The standard curves were linear over the range of 0.05-20μgmL−1 for hydrochlorothiazide and 0.02-5.0μgmL−1 for reserpine, respectively (r>0.999). The limit of detection (LOD) and the limit of quantification (LOQ) were 5.5 and 18.2ngmL−1 for hydrochlorothiazide, and 7.1 and 23.6ngmL−1 for reserpine, respectively. The recoveries for both analytes were above 89±1.35%. The intra-day and inter-day precision for hydrochlorothiazide were less than 1.91 and 1.38%, and those for reserpine were below 1.61 and 2.64%, respectively. The method indicated good performance in terms of specificity, linearity, detection and quantification limits, precision and accuracy, and was employed successfully for the simultaneous determination of hydrochlorothiazide and reserpine in human urine samples. Springer-Verlag, 2011 Column liquid chromatography nationallicence Sample pre-treatment nationallicence Hydrochlorothiazide and reserpine in urine nationallicence Li Hang Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut He Junting Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut Liu Qin Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut Huo Zhaohui Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut Liang Si Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut Liang Yong Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut Ito Yoichiro Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Hear, Lung and Blood Institutes, National Institutes of Health, Bethesda, MD, USA aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 171-175 0009-5893 73:1-2<171 2011 73 10337 https://doi.org/10.1007/s10337-010-1821-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1821-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Hang Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- He Junting Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Qin Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huo Zhaohui Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Si Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Yong Institute of Analytical Chemistry, School of Chemistry and Environment, South China Normal University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ito Yoichiro Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Hear, Lung and Blood Institutes, National Institutes of Health, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 171-175 0009-5893 73:1-2<171 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer