caa a22 4500 445802219 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1835-z doi (NATIONALLICENCE)springer-10.1007/s10337-010-1835-z A Simple Method for Monitoring Iodinated Casein in Feed Premixes by LC-MS Using Iodothyronines as Markers [Elektronische Daten] [Zongyi Wang, Wenjun Yang, Biying Chang, Xiangning Chen, Liying Zhang, Lihua He] A simple and effective liquid chromatography-mass spectrometry method was devoleped for mornitoring iodinated casein (IC) in feed premixes. In this method, iodothyronines released from IC upon sample hydrolysis were used as markers. Samples were treated with Na2S and hydrolyzed with Ba(OH)2·H2O followed by cleaned up using a SampliQ SPE cartridge. Chromatographic separation was achieved on a C18 column with isocratic elution using water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Ion detection was performed using electrospray ionization in positive mode using selected ion monitoring at m/z 606, 652, 732 and 778. IC levels were mornitored by qualitative analysis of the iodothyronines and quantification of thyroxine. Validation data demonstrated that the method was selective and sensitive (≤0.5mgg−1) with acceptable accuracy (70.2-128.2% recovery) and precison (RSD 5.5-15.2%). Springer-Verlag, 2011 Column liquid chromatography-mass spectrometry nationallicence Iodothyronine nationallicence Iodinated casein nationallicence Feed premix nationallicence Wang Zongyi State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut Yang Wenjun State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut Chang Biying State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut Chen Xiangning Faculty of Food Science, Beijing University of Agricluture, 102206, Beijing, People's Republic of China aut Zhang Liying State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut He Lihua State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 143-147 0009-5893 73:1-2<143 2011 73 10337 https://doi.org/10.1007/s10337-010-1835-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1835-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zongyi State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yang Wenjun State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chang Biying State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiangning Faculty of Food Science, Beijing University of Agricluture, 102206, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Liying State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- He Lihua State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 143-147 0009-5893 73:1-2<143 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer