caa a22 4500 445802227 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1832-2 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1832-2 Analysis of Amisulpride in Human Plasma by SPE and LC with Fluorescence Detection [Elektronische Daten] [Illia Kudris, Nona Skakun, Irina Orlova, Victoria Libina, Artem Kulikov] A rapid, precise, accurate, and selective high-performance liquid chromatographic method with fluorescence detection has been validated and used for analysis of amisulpride in human plasma after a simple solid-phase extraction procedure. Compounds were separated on a CN column with 0.03M potassium dihydrogen phosphate (pH 6.5)-acetonitrile 65:35 (v/v) as mobile phase. Fluorescence detection was performed at excitation and emission wavelengths of 274 and 370nm, respectively. Calibration plots were linear over the concentration range 10-1,000ngmL−1 in human plasma, and the lower limit of quantification was 10ngmL−1. Accuracy was between 0.4 and 6.4% and precision was between 3.1 and 7.5%. Amisulpride was sufficiently stable through three freeze-thaw cycles, during storage for 6h at room temperature, and for 2months at −22°C. The method is suitable for the analysis of clinical samples from pharmacokinetic studies. The Author(s), 2011 Column liquid chromatography nationallicence Amisulpride nationallicence Validation nationallicence Solid-phase extraction nationallicence Pharmacokinetic study nationallicence Kudris Illia Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut Skakun Nona Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut Orlova Irina Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut Libina Victoria Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut Kulikov Artem Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 67-74 0009-5893 73:1-2<67 2011 73 10337 https://doi.org/10.1007/s10337-010-1832-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1832-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kudris Illia Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut NATIONALLICENCE 700 1- Skakun Nona Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut NATIONALLICENCE 700 1- Orlova Irina Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut NATIONALLICENCE 700 1- Libina Victoria Laboratory of Pharmacokinetics, State Pharmacological Center, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut NATIONALLICENCE 700 1- Kulikov Artem Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 67-74 0009-5893 73:1-2<67 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer