caa a22 4500 445802235 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1825-1 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1825-1 A Rapid and Sensitive Assay for Determining the Main Components in Processed Fructus Corni by UPLC-Q-TOF-MS [Elektronische Daten] [Gang Cao, Yun Zhang, Jian Feng, Hao Cai, Chengrong Zhang, Mingjian Ding, Xiaodong Cong, Baochang Cai] Fructus Corni is the dried sarcocarp of Cornus officinalis Sieb. et Zucc., and has been widely used as an important traditional Chinese medicine, which is used to nourish liver and kidney conditions and to astringe semen as well as to solidify collapse. Precise structural identification of the main compounds in processed Fructus Corni has been established using ultra performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight-mass spectrometry (Q-TOF-MS), and BEH C18 column for direct analysis of total water extracts. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode. The MS data for candidates, containing accurate mass for both precursors and their fragment ions, were acquired selectively. Based on the MS data, the mass spectrometric fingerprint (MSFP) for candidates, consisting of chemical formula and dissociation pattern, was determined. By this method, eight iridoid glycosides and seven other compounds were identified or tentatively identified. Therefore, MS combined with selective enrichment provided a powerful means for analyzing main compounds in processed Fructus Corni. LC-Q-TOF-MS based chemical profiling is a rapid and powerful approach for holistic quality evaluation of processed Fructus Corni, and should also be useful for the global quality investigation of decoctions derived from other herbal medicines. Springer-Verlag, 2011 Ultra performance liquid chromatography nationallicence Quadropole-time-of-flight-mass-spectrometry nationallicence Iridoid glycosides nationallicence Processed Fructus Corni nationallicence Cao Gang Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Zhang Yun Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Feng Jian Department of Pharmaceutics, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Cai Hao Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 210029, Nanjing, People's Republic of China aut Zhang Chengrong Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Ding Mingjian Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Cong Xiaodong Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Cai Baochang Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 135-141 0009-5893 73:1-2<135 2011 73 10337 https://doi.org/10.1007/s10337-010-1825-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1825-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cao Gang Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yun Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Feng Jian Department of Pharmaceutics, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cai Hao Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chengrong Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ding Mingjian Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cong Xiaodong Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cai Baochang Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, 310053, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 135-141 0009-5893 73:1-2<135 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer