caa a22 4500 445802243 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1833-1 doi (NATIONALLICENCE)springer-10.1007/s10337-010-1833-1 Simultaneous LC-UV-MS-MS Analysis of Nine Pivotal Metabolites in Human Serum: Application to Studies of Impaired Glucose Tolerance [Elektronische Daten] [Hongfu Zhong, Qionglin Liang, Jianfei Xia, Ping Hu, Yiming Wang, Xiaolin Tong, Guoan Luo] A rapid, sensitive, and selective LC-UV-MS-MS method for simultaneous quantification of uric acid, creatinine, xanthine, creatine, hypoxanthine, adenosine, inosine, thymidine, and uridine in serum from patients with impaired glucose tolerance (IGT) has been developed and validated. After precipitation of protein in the serum with methanol samples were evaporated with a stream of nitrogen then reconstituted with aqueous ammonia (0.002molL−1). Chromatographic separation was performed on a C18 column with methanol-buffer (8mmolL−1 ammonium acetate adjusted to pH 6.1 with glacial acetic acid) as mobile phase at a flow rate of 0.8mLmin−1. UV and MS-MS detection were used to quantify the metabolites on the basis of different concentrations and detector response. Linearity was excellent, with r 2 no <0.998. Recovery, and intra-day and inter-day relative standard deviation (RSD) were >85, <10, and <10%, respectively. This reliable bioanalytical method enables evaluation of the levels of purines and pyrimidines in serum for IGT studies and diagnosis. Springer-Verlag, 2011 Column liquid chromatography nationallicence Tandem mass spectrometry nationallicence Human serum nationallicence Purines and pyrimidines nationallicence Impaired glucose tolerance nationallicence Zhong Hongfu School of Pharmaceutics, East-China University of Science and Technology, 200237, Shanghai, China aut Liang Qionglin Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut Xia Jianfei School of Pharmaceutics, East-China University of Science and Technology, 200237, Shanghai, China aut Hu Ping College of Chemical and Molecular Engineering, East-China University of Science and Technology, 200237, Shanghai, China aut Wang Yiming Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut Tong Xiaolin China Academy of Chinese Medical Sciences, Guang'anmen Hospital, 100053, Beijing, China aut Luo Guoan Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 149-155 0009-5893 73:1-2<149 2011 73 10337 https://doi.org/10.1007/s10337-010-1833-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1833-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhong Hongfu School of Pharmaceutics, East-China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Liang Qionglin Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Xia Jianfei School of Pharmaceutics, East-China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Hu Ping College of Chemical and Molecular Engineering, East-China University of Science and Technology, 200237, Shanghai, China aut NATIONALLICENCE 700 1- Wang Yiming Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Tong Xiaolin China Academy of Chinese Medical Sciences, Guang'anmen Hospital, 100053, Beijing, China aut NATIONALLICENCE 700 1- Luo Guoan Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 149-155 0009-5893 73:1-2<149 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer