caa a22 4500 445802294 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10337-010-1837-x doi (NATIONALLICENCE)springer-10.1007/s10337-010-1837-x UPLC a Powerful Tool for the Separation of Imidazolium Ionic Liquid Cations [Elektronische Daten] [Asta Orentienė, Vilma Olšauskaitė, Vida Vičkačkaitė, Audrius Padarauskas] Ultra-performance liquid chromatography (UPLC) in reversed-phase (RP), ion pair (IP) and hydrophilic interaction chromatography (HILIC) has been investigated for the separation of imidazolium-based ionic liquid (IL) cations. Among the three stationary phases (i.e., C18, C8 and phenyl) studied under RP conditions the phenyl phase provided much stronger retention for the IL cations. Four acids (hydrochloric, methanesulfonic, perchloric and trifluoroacetic) as mobile phase additives were compared in light of their effects on the retention of IL cations. It was shown that the retention of all IL cations decreased upon acidification of the mobile phase, possibly due to suppression of residual silanol ionization. Very fast (~3min) and efficient RP-UPLC separation of six cations was achieved by gradient elution with acetonitrile-water mobile phase containing 2.5mmolL−1 perchloric acid. In IP-UPLC all solutes were well resolved in about 4min by gradient elution with acetonitrile-water mobile phase containing 1mmolL−1 sodium 1-octanesulfonate as ion pairing reagent. Finally, under HILIC conditions by using isocratic elution with acetonitrile-water (85:15, v/v) mobile phase containing 5mmol L−1 ammonium formate (pH 3.2) the separation time was reduced to less than 2min while maintaining excellent peak shapes and sufficient resolution. Compared to current LC systems UPLC allowed considerably faster separations with better peak shapes. Springer-Verlag, 2011 Ultra-performance liquid chromatography nationallicence Reversed-phase chromatography nationallicence Ion pair chromatography nationallicence Hydrophilic interaction chromatography nationallicence Ionic liquids nationallicence Imidazolium cations nationallicence Orentienė Asta Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Olšauskaitė Vilma Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Vičkačkaitė Vida Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Padarauskas Audrius Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 17-24 0009-5893 73:1-2<17 2011 73 10337 https://doi.org/10.1007/s10337-010-1837-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-010-1837-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orentienė Asta Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Olšauskaitė Vilma Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Vičkačkaitė Vida Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Padarauskas Audrius Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius, Lithuania aut NATIONALLICENCE 773 0- Chromatographia Vieweg Verlag 73/1-2(2011-01-01), 17-24 0009-5893 73:1-2<17 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer