caa a22 4500 44580243X CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2036-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2036-0 Rapid, Sensitive and Selective Analysis of trans -Resveratrol in Rat Plasma by LC-MS-MS [Elektronische Daten] [Meng-xiang Su, Bin Di, Tai-jun Hang, Jun Wang, Da-song Yang, Tai-hu Wang, Rui Meng] A rapid, sensitive and highly selective liquid chromatography/tandem mass spectrometry (LC-MS-MS) using an atmospheric pressure chemical ionization (APCI) method was developed to determine the concentration of trans-resveratrol in rat plasma. Samples were extracted using ethyl acetate after daidzein was added as an internal standard (IS). The main advantages of this method were the high sensitivity (a lower limit of quantification, LLOQ, of 0.1ngmL−1) and the short analysis time (total run time 3.2min per sample). Separation was performed on a ZORBAX ODS column with a mobile phase that consisted of acetonitrile/water (48:52, v/v). trans-Resveratrol and IS were detected using a triple-quadrupole mass spectrometer in the selective reaction monitoring (SRM) mode with the parent-to-product quantifier transitions [M-H]− m/z 226.8→142.9 and [M-H]− m/z 252.9→252.9 for trans-resveratrol and IS, respectively. The method was confirmed to be accurate and precise with a linearity range of 0.1-500ngmL−1 with r>0.99. Recoveries for trans-resveratrol and IS were within 90-101%. The accuracy and precision for the assay were determined by calculating the intra- and inter-batch variation for quality control (QC) samples at four concentration levels with a relative standard deviation (RSD) of <15%. This method was successfully applied to determine trans-resveratrol in rat plasma and proved suitable for pharmacokinetic study after intragastric administration of trans-resveratrol in sustained release granules and normal granules, respectively. Springer-Verlag, 2011 Column liquid chromatography nationallicence Mass spectrometry nationallicence trans -Resveratrol nationallicence Rat plasma nationallicence Su Meng-xiang Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut Di Bin Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut Hang Tai-jun Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut Wang Jun Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut Yang Da-song Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut Wang Tai-hu Nanjing Potomac Bio-technology Co., Ltd, 211800, Nanjing, China aut Meng Rui Nanjing Potomac Bio-technology Co., Ltd, 211800, Nanjing, China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1203-1210 0009-5893 73:11-12<1203 2011 73 10337 https://doi.org/10.1007/s10337-011-2036-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2036-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Su Meng-xiang Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Di Bin Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Hang Tai-jun Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Wang Jun Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Yang Da-song Department of Pharmaceutical Analysis, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Wang Tai-hu Nanjing Potomac Bio-technology Co., Ltd, 211800, Nanjing, China aut NATIONALLICENCE 700 1- Meng Rui Nanjing Potomac Bio-technology Co., Ltd, 211800, Nanjing, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1203-1210 0009-5893 73:11-12<1203 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer