caa a22 4500 445802456 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2011-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2011-9 Simultaneous Determination of Pethidine and Atropine in Rabbit Plasma by LC-MS-MS and Its Application to a Pharmacokinetic Study after Intramuscular Administration [Elektronische Daten] [Chan Chen, Jie Deng, Shougang Wang, Jiejin Zhu, Meifei Lu, Hongzhang He, Yinping Fu, Xianqin Wang, Jianshe Ma] A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of pethidine and atropine in rabbit plasma was developed and validated. The analytes and internal standard (IS) are extracted from plasma by liquid-liquid extraction using ethyl acetate, and separated on a Zorbax SB-Aq column (2.1×150mm, 3.5μm) using acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization source was applied and operated in positive ion mode, and multiple reaction monitoring mode was used for quantification using target fragment ions m/z 247.8→219.7 for pethidine, m/z 289.9→123.8 for atropine and m/z 295.0→266.8 for IS, respectively. The assay is linear over the range of 5-1,000ngmL−1 for pethidine and atropine, with a lower limit of quantification of 3ngmL−1 for pethidine and 5ngmL−1 for atropine. Intra-day and inter-day precision are less than 11% and the accuracy are in the range of 90.4-106.3%. Furthermore, the newly developed method is successfully used for the determination of pethidine and atropine in rabbit plasma for pharmacokinetic study. Springer-Verlag, 2011 Column liquid chromatography-tandem mass spectrometry nationallicence Pethidine nationallicence Atropine nationallicence Intramuscular nationallicence Plasma nationallicence Chen Chan The First Affiliated Hospital of Wenzhou Medical College, 325035, Wenzhou, China aut Deng Jie Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut Wang Shougang Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China aut Zhu Jiejin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Lu Meifei Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut He Hongzhang Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Fu Yinping Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Wang Xianqin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut Ma Jianshe Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1197-1202 0009-5893 73:11-12<1197 2011 73 10337 https://doi.org/10.1007/s10337-011-2011-9 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2011-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Chan The First Affiliated Hospital of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Deng Jie Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Wang Shougang Institute of Plant Nutrition, Agricultural Resources and Environmental Science, Henan Academy of Agricultural Sciences, 450002, Zhengzhou, China aut NATIONALLICENCE 700 1- Zhu Jiejin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Lu Meifei Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- He Hongzhang Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Fu Yinping Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Wang Xianqin Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 700 1- Ma Jianshe Function Experiment Teaching Center of Wenzhou Medical College, 325035, Wenzhou, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1197-1202 0009-5893 73:11-12<1197 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer