caa a22 4500 445802472 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2000-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2000-z Determination of 20( R )-25-Methoxyl-dammarane-3,12,20-triol and Its Active Metabolite in Beagle Dog Plasma by LC-MS-MS and Its Application to Pharmacokinetics Studies [Elektronische Daten] [Xingchao Liu, Youping Liu, Xin Di, Xin Wang, Chunhong Zhang, Changxiao Liu] A rapid and sensitive liquid chromatography-tandem mass spectrometric method (LC-MS-MS) has been developed for simultaneous determination of 20(R)-25-methoxyl-dammarane-3,12,20-triol (25-OCH3-PPD) and its active metabolite, 20(R)-dammarane-3,12,20,25-tetrol (25-OH-PPD) in beagle dog's plasma. Diazepam was employed as an internal standard. Sample preparation involved a liquid-liquid extraction with diethyl ether and dichloromethane (3:2, v/v). Chromatographic separation was achieved on a Phenomenex C18 material (50×2.0mm i.d., 4μm) using methanol-water-formic acid (124:26:0.3, v/v/v) as the mobile phase at a flow rate of 0.25mLmin−1. The present method exhibited good linearity over the concentration range of 2-500ngmL−1 for 25-OCH3-PPD and 2-60ngmL−1 for 25-OH-PPD. The lower limit of quantification was 2 and 2ngmL−1 for 25-OCH3-PPD and 25-OH-PPD, respectively. The intra- and inter-day precision values for 25-OCH3-PPD and 25-OH-PPD met the requirements of the FDA guidance, and the accuracy (RE) ranged from −4.1 to 5.6% calculated from QC samples. No endogenous compound was found to interfere with the analysis. 25-OCH3-PPD and 25-OH-PPD were stable during all storage, pretreatment and analytical periods. The validated method was successfully used to study the pharmacokinetics of 25-OCH3-PPD in beagle dogs after oral administration at a dose of 12mgkg−1. Springer-Verlag, 2011 LC-MS-MS nationallicence 20( R )-25-Methoxyl-dammarane-3,12,20-triol nationallicence 20( R )-dammarane-3,12,20,25-tetrol nationallicence Pharmacokinetics nationallicence Liu Xingchao Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Liu Youping Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Di Xin Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Wang Xin Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Zhang Chunhong Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Liu Changxiao Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, 308 An Shan West Road, 300193, Tianjin, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1105-1110 0009-5893 73:11-12<1105 2011 73 10337 https://doi.org/10.1007/s10337-011-2000-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2000-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Xingchao Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Youping Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Di Xin Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xin Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chunhong Laboratory of Drug Metabolism and Pharmacokinetics, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Changxiao Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, 308 An Shan West Road, 300193, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1105-1110 0009-5893 73:11-12<1105 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer