caa a22 4500 445802480 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2010-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-2010-x Determination of Tetrandrine in Rat Whole Blood by RP-LC and Its Application to Pharmacokinetic Studies [Elektronische Daten] [Ying Zhang, Ling Wang, Han Zhang, Xuehua Jiang, Qingqing Du] A simple, specific and sensitive RP-LC method was developed and validated for the determination of tetrandrine in rat whole blood for the first time. Chromatographic separation was performed on a WelchromTM C18 analytical column at a flow rate of 1.0mLmin−1, using a mixture of methanol-water containing 0.6% triethylamine and 0.16% phosphoric acid as mobile phase. The wavelength for UV detection was set at 225nm. Sample preparation involved a liquid-liquid extraction using n-hexane. The calibration curve was linear with r 2>0.99 over a concentration range of 0.03-6.4μgmL−1 in rat whole blood with a lower limit of quantification of 0.03μgmL−1. The intra- and inter-day precisions were 1.33-4.55 and 3.33-4.65%, respectively, and the intra- and inter-day accuracy ranged from −5.24 to 0.90% and −1.05 to 0.63%, respectively. No endogenous compounds were found to interfere with the analytes. Tetrandrine was stable for 8h at room temperature, 24h at 4°C in rat whole blood, and for 30 days at −20°C after being prepared. For the first time, the present method was successfully applied to the pharmacokinetic studies of tetrandrine in rats after intravenous administration of three different doses. The results indicated that the pharmacokinetics of tetrandrine on rats was a first-order process. Springer-Verlag, 2011 Reversed phase liquid chromatography nationallicence Tetrandrine nationallicence Pharmacokinetics nationallicence Whole blood concentration nationallicence Zhang Ying The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut Wang Ling The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut Zhang Han The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut Jiang Xuehua The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut Du Qingqing The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1097-1103 0009-5893 73:11-12<1097 2011 73 10337 https://doi.org/10.1007/s10337-011-2010-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2010-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ying The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Ling The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Han The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Xuehua The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Du Qingqing The Department of Clinical Pharmacy and Pharmacy Administration, West China School of Pharmacy, Sichuan University, No. 17, Section3, Southern Renmin Road, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1097-1103 0009-5893 73:11-12<1097 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer